Genetic Transformation of Date Palm Via Microprojectile Bombardment.
Methods Mol Biol
; 1637: 269-280, 2017.
Article
in En
| MEDLINE
| ID: mdl-28755352
ABSTRACT
Efficient protocols for date palm embryogenic callus and somatic embryo transformation with uidA gene are described in this chapter. The embryogenic callus transformation procedure is 1.6 µm gold particle size coated with 2.5 µg DNA (pAct1-D plasmid), 1100 psi helium pressure, 9 cm target distance, 26 inHg vacuum pressure, 3 mm distance between the rupture disk and macrocarrier, and osmotic pretreatment with 0.4 M mannitol followed by 60 min air desiccation. The somatic embryo transformation procedure is 0.6 µm gold particle size coated with 2.5 µg DNA (pAct1-D plasmid), 1350 psi helium pressure, 6 cm target distance, 28 inHg vacuum pressure, 3 mm distance between the rupture disk and macrocarrier, and osmotic pretreatment with 0.4 M mannitol followed by 60 min air desiccation. Protocols for analysis of the transgenic plantlets have also been described.
Key words
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Plants, Genetically Modified
/
Biolistics
/
Phoeniceae
Language:
En
Journal:
Methods Mol Biol
Journal subject:
BIOLOGIA MOLECULAR
Year:
2017
Document type:
Article
Affiliation country:
Iran