A streamlined method for analysing genome-wide DNA methylation patterns from low amounts of FFPE DNA.

Ludgate, Jackie L; Wright, James; Stockwell, Peter A; Morison, Ian M; Eccles, Michael R; Chatterjee, Aniruddha

Ludgate, Jackie L; Wright, James; Stockwell, Peter A; Morison, Ian M; Eccles, Michael R; Chatterjee, Aniruddha.

Affiliation

Ludgate JL; Department of Pathology, Dunedin School of Medicine, University of Otago, 270 Great King Street, P.O. Box 56, Dunedin, 9054, New Zealand.
Wright J; Department of Pathology, Dunedin School of Medicine, University of Otago, 270 Great King Street, P.O. Box 56, Dunedin, 9054, New Zealand.
Stockwell PA; School of Biosciences, Cardiff University, Sir Martin Evans Building, Museum Avenue, Cardiff, CF10 3AX, UK.
Morison IM; Department of Biochemistry, University of Otago, 710 Cumberland Street, Dunedin, 9054, New Zealand.
Eccles MR; Department of Pathology, Dunedin School of Medicine, University of Otago, 270 Great King Street, P.O. Box 56, Dunedin, 9054, New Zealand.
Chatterjee A; Department of Pathology, Dunedin School of Medicine, University of Otago, 270 Great King Street, P.O. Box 56, Dunedin, 9054, New Zealand.

BMC Med Genomics ; 10(1): 54, 2017 08 31.

Article in En | MEDLINE | ID: mdl-28859641

Subject(s)

DNA Methylation; DNA/genetics; Formaldehyde; Genomics/methods; Paraffin Embedding; Tissue Fixation; High-Throughput Nucleotide Sequencing; Sequence Analysis, DNA

Key words

Bisulfite sequencing; DNA extraction; DNA methylation; FFPE tissue; Genome-wide; RRBS

Fulltext

Add to My VHL

XML

PubMed Links

Search on Google

Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: DNA / Tissue Fixation / Paraffin Embedding / DNA Methylation / Genomics / Formaldehyde Type of study: Guideline Language: En Journal: BMC Med Genomics Journal subject: GENETICA MEDICA Year: 2017 Document type: Article Affiliation country: New Zealand Country of publication: United kingdom

Fulltext

Add to My VHL

XML

PubMed Links

Search on Google