Site-specific chromosomal gene insertion: Flp recombinase versus Cas9 nuclease.

Phan, Quang Vinh; Contzen, Jörg; Seemann, Petra; Gossen, Manfred

Phan, Quang Vinh; Contzen, Jörg; Seemann, Petra; Gossen, Manfred.

Affiliation

Phan QV; Berlin-Brandenburg Center for Regenerative Therapies (BCRT), Föhrer Strasse 15, 13353, Berlin, Germany.
Contzen J; Helmholtz-Zentrum Geesthacht (HZG), Institute of Biomaterial Science, Teltow, 14513, Germany.
Seemann P; Charité - Universitätsmedizin Berlin, Labor für Pädiatrische Molekularbiologie, Berlin, Germany.
Gossen M; Berlin-Brandenburg Center for Regenerative Therapies (BCRT), Föhrer Strasse 15, 13353, Berlin, Germany.

Sci Rep ; 7(1): 17771, 2017 12 19.

Article in En | MEDLINE | ID: mdl-29259215

Subject(s)

CRISPR-Associated Protein 9/genetics; Chromosomes/genetics; DNA Nucleotidyltransferases/genetics; Mutagenesis, Insertional/genetics; Recombination, Genetic/genetics; Animals; CHO Cells; CRISPR-Cas Systems/genetics; Cell Line; Clustered Regularly Interspaced Short Palindromic Repeats/genetics; Cricetulus; HEK293 Cells; Humans; Transgenes/genetics

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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Recombination, Genetic / Mutagenesis, Insertional / Chromosomes / DNA Nucleotidyltransferases / CRISPR-Associated Protein 9 Limits: Animals / Humans Language: En Journal: Sci Rep Year: 2017 Document type: Article Affiliation country: Germany Country of publication: United kingdom

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