Botulinum hemagglutinin-mediated in situ break-up of human induced pluripotent stem cell aggregates for high-density suspension culture.

Nath, Suman C; Tokura, Tomohiro; Kim, Mee-Hae; Kino-Oka, Masahiro

Nath, Suman C; Tokura, Tomohiro; Kim, Mee-Hae; Kino-Oka, Masahiro.

Affiliation

Nath SC; Department of Biotechnology, Graduate School of Engineering, Osaka University, Suita, Osaka, Japan.
Tokura T; Department of Biotechnology, Graduate School of Engineering, Osaka University, Suita, Osaka, Japan.
Kim MH; Fujimori Kogyo Co. Ltd., Shinjuku, Tokyo, Japan.
Kino-Oka M; Department of Biotechnology, Graduate School of Engineering, Osaka University, Suita, Osaka, Japan.

Biotechnol Bioeng ; 115(4): 910-920, 2018 04.

Article in En | MEDLINE | ID: mdl-29278408

Subject(s)

Cell Communication/drug effects; Cell Culture Techniques; Clostridium botulinum/metabolism; Hemagglutinins/pharmacology; Induced Pluripotent Stem Cells/drug effects; Antigens, CD/metabolism; Bioreactors; Cadherins/metabolism; Cell Aggregation/drug effects; Cell Count; Culture Media/analysis; Humans; Induced Pluripotent Stem Cells/cytology; Kinetics

Key words

botulinum hemagglutinin; high-density culture; human induced pluripotent stem cells; in situ aggregate break-up; suspension culture; temporal E-cadherin disruption

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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Cell Communication / Clostridium botulinum / Cell Culture Techniques / Induced Pluripotent Stem Cells / Hemagglutinins Limits: Humans Language: En Journal: Biotechnol Bioeng Year: 2018 Document type: Article Affiliation country: Japan Country of publication: United States

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