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Generation of patient-specific induced pluripotent stem cells from Leber's hereditary optic neuropathy.
Lu, Huai-En; Yang, Yi-Ping; Chen, Yan-Ting; Wu, You-Ren; Wang, Chia-Lin; Tsai, Fu-Ting; Hwang, De-Kuang; Lin, Tai-Chi; Chen, Shih-Jen; Wang, An-Guor; Hsieh, Patrick C H; Chiou, Shih-Hwa.
Affiliation
  • Lu HE; Bioresource Collection and Research Center, Food Industry Research and Development Institute, Hsinchu, Taiwan.
  • Yang YP; School of Medicine, National Yang-Ming University, Taipei, Taiwan; Department of Neurosurgery, Tri-Service General Hospital, National Defense Medical Center, Taipei, Taiwan.
  • Chen YT; Department of Ophthalmology, Changhua Christian Hospital, Changhua, Taiwan; Institute of Clinical Medicine, National Yang-Ming University, Taipei, Taiwan; Department of Optometry, Central Taiwan University of Science and Technology, Taichung, Taiwan.
  • Wu YR; Institute of Pharmacology, National Yang-Ming University, Taipei, Taiwan.
  • Wang CL; Department of Medical Research, Taipei Veterans General Hospital, Taipei, Taiwan.
  • Tsai FT; Department of Medical Research, Taipei Veterans General Hospital, Taipei, Taiwan.
  • Hwang DK; Department of Ophthalmology, Taipei Veterans General Hospital, Taipei, Taiwan; School of Medicine, National Yang-Ming University, Taipei, Taiwan.
  • Lin TC; Department of Ophthalmology, Taipei Veterans General Hospital, Taipei, Taiwan; Institute of Clinical Medicine, National Yang-Ming University, Taipei, Taiwan.
  • Chen SJ; Department of Ophthalmology, Taipei Veterans General Hospital, Taipei, Taiwan; School of Medicine, National Yang-Ming University, Taipei, Taiwan.
  • Wang AG; Department of Ophthalmology, Taipei Veterans General Hospital, Taipei, Taiwan; School of Medicine, National Yang-Ming University, Taipei, Taiwan.
  • Hsieh PCH; Institute of Biomedical Sciences, Academia Sinica, Taipei, Taiwan.
  • Chiou SH; Institute of Pharmacology, National Yang-Ming University, Taipei, Taiwan; Department of Medical Research, Taipei Veterans General Hospital, Taipei, Taiwan; Genomic Research Center, Academia Sinica, Taipei, Taiwan. Electronic address: shchiou@vghtpe.gov.tw.
Stem Cell Res ; 28: 56-60, 2018 04.
Article in En | MEDLINE | ID: mdl-29427840
Leber's hereditary optic neuropathy (LHON) is a maternally inherited mitochondrial disease caused by homoplasmic point mutations in complex I subunit genes of mitochondrial DNA. In this report, we generated an induced pluripotent stem cell (iPSCs) line, TVGH-iPSC-010-09, from the peripheral blood mononuclear cells of a female patient with Leber's hereditary optic neuropathy (LHON) by using the Sendai-virus delivery system. The resulting iPSCs retained the disease-causing mitochondrial DNA mutation, expressed pluripotent markers and could differentiate into the three germ layers. We believe LHON patient-specific iPSCs provide a powerful in vitro model for evaluating the pathological phenotypes of the disease.
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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Cell Culture Techniques / Optic Atrophy, Hereditary, Leber / Induced Pluripotent Stem Cells Limits: Animals / Female / Humans / Middle aged Language: En Journal: Stem Cell Res Year: 2018 Document type: Article Affiliation country: Taiwan Country of publication: United kingdom
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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Cell Culture Techniques / Optic Atrophy, Hereditary, Leber / Induced Pluripotent Stem Cells Limits: Animals / Female / Humans / Middle aged Language: En Journal: Stem Cell Res Year: 2018 Document type: Article Affiliation country: Taiwan Country of publication: United kingdom