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1,8-Cineole blocks voltage-gated L-type calcium channels in tracheal smooth muscle.
Pereira-Gonçalves, Átila; Ferreira-da-Silva, Francisco Walber; de Holanda-Angelin-Alves, Camille Maria; Cardoso-Teixeira, Ana Carolina; Coelho-de-Souza, Andrelina Noronha; Leal-Cardoso, José Henrique.
Affiliation
  • Pereira-Gonçalves Á; Laboratory of Electrophysiology and Muscular Contraction, Superior Institute of Biomedical Sciences, State University of Ceará, Campus of Itaperi, Fortaleza, CE, Brazil.
  • Ferreira-da-Silva FW; Laboratory of Electrophysiology and Muscular Contraction, Superior Institute of Biomedical Sciences, State University of Ceará, Campus of Itaperi, Fortaleza, CE, Brazil.
  • de Holanda-Angelin-Alves CM; Civil Engineering Department, State University of Vale do Acaraú, Sobral, CE, Brazil.
  • Cardoso-Teixeira AC; Laboratory of Electrophysiology and Muscular Contraction, Superior Institute of Biomedical Sciences, State University of Ceará, Campus of Itaperi, Fortaleza, CE, Brazil.
  • Coelho-de-Souza AN; Laboratory of Electrophysiology and Muscular Contraction, Superior Institute of Biomedical Sciences, State University of Ceará, Campus of Itaperi, Fortaleza, CE, Brazil.
  • Leal-Cardoso JH; Laboratory of Electrophysiology and Muscular Contraction, Superior Institute of Biomedical Sciences, State University of Ceará, Campus of Itaperi, Fortaleza, CE, Brazil.
Pflugers Arch ; 470(12): 1803-1813, 2018 12.
Article in En | MEDLINE | ID: mdl-30206706
1,8-Cineole is a cyclic monoterpenoid used in folk medicine for treatment of numerous respiratory diseases and other infections. 1,8-Cineole has anti-inflammatory, antioxidant, and myorelaxant effects, as well as low toxicity. In the present study, the effects of 1,8-cineole on contractility and voltage-gated calcium channels (VGCC) in tracheal smooth muscle were investigated. Intact and dissociated tracheal smooth muscle were used for muscle contraction and patch-clamp recordings, respectively. In experiments involving muscle contraction, 1,8-cineole potentiated contractions at low concentrations and relaxed contractions induced by isotonic K+ at high concentrations. AMTB (a TRPM8 channel blocker) reduced the potentiation induced by 1,8-cineole while indomethacin (a COX inhibitor) did not block this effect. In dissociated myocytes, 1,8-cineole partially blocked Ba2+ currents through VGCC in a concentration-dependent manner. 1,8-Cineole shifted the steady-state activation and inactivation curves to the left and also reduced the current decay time constant. In conclusion, 1,8-cineole has a dual effect on tracheal smooth muscle contraction resulting in a biphasic effect. Our data suggest that the potentiation effect is mediated by activation of TRPM8 channels and the relaxation effect is mediated by the blockage of L-type VGCC.
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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Trachea / Calcium Channel Blockers / Calcium Channels, L-Type / Myocytes, Smooth Muscle / Eucalyptol Limits: Animals Language: En Journal: Pflugers Arch Year: 2018 Document type: Article Affiliation country: Brazil Country of publication: Germany

Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Trachea / Calcium Channel Blockers / Calcium Channels, L-Type / Myocytes, Smooth Muscle / Eucalyptol Limits: Animals Language: En Journal: Pflugers Arch Year: 2018 Document type: Article Affiliation country: Brazil Country of publication: Germany