Combinatorial pathway enzyme engineering and host engineering overcomes pyruvate overflow and enhances overproduction of N-acetylglucosamine in Bacillus subtilis.

Ma, Wenlong; Liu, Yanfeng; Lv, Xueqin; Li, Jianghua; Du, Guocheng; Liu, Long

Ma, Wenlong; Liu, Yanfeng; Lv, Xueqin; Li, Jianghua; Du, Guocheng; Liu, Long.

Affiliation

Ma W; Key Laboratory of Carbohydrate Chemistry and Biotechnology, Ministry of Education, Jiangnan University, Wuxi, 214122, China.
Liu Y; Key Laboratory of Industrial Biotechnology, Ministry of Education, Jiangnan University, Wuxi, 214122, China.
Lv X; Key Laboratory of Carbohydrate Chemistry and Biotechnology, Ministry of Education, Jiangnan University, Wuxi, 214122, China.
Li J; Key Laboratory of Industrial Biotechnology, Ministry of Education, Jiangnan University, Wuxi, 214122, China.
Du G; Key Laboratory of Carbohydrate Chemistry and Biotechnology, Ministry of Education, Jiangnan University, Wuxi, 214122, China.
Liu L; Key Laboratory of Industrial Biotechnology, Ministry of Education, Jiangnan University, Wuxi, 214122, China.

Microb Cell Fact ; 18(1): 1, 2019 Jan 04.

Article in En | MEDLINE | ID: mdl-30609921

Subject(s)

Acetylglucosamine/metabolism; Bacillus subtilis/metabolism; Caenorhabditis elegans Proteins/metabolism; Glucosamine 6-Phosphate N-Acetyltransferase/metabolism; Metabolic Engineering; Pyruvic Acid/metabolism; Acetoin/metabolism; Animals; Bacillus subtilis/enzymology; Bacillus subtilis/growth & development; Bacterial Proteins/genetics; Bacterial Proteins/metabolism; Batch Cell Culture Techniques; Caenorhabditis elegans/enzymology; Caenorhabditis elegans Proteins/genetics; Glucosamine 6-Phosphate N-Acetyltransferase/genetics; Glucose/metabolism; Hydrogen-Ion Concentration; Kinetics; Urease/genetics; Urease/metabolism

Key words

Bacillus subtilis; Glucosamine-6-phosphate N-acetyltransferase; N-Acetylglucosamine; Overflow; Pyruvate; Urease

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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Acetylglucosamine / Bacillus subtilis / Pyruvic Acid / Caenorhabditis elegans Proteins / Glucosamine 6-Phosphate N-Acetyltransferase / Metabolic Engineering Limits: Animals Language: En Journal: Microb Cell Fact Journal subject: BIOTECNOLOGIA / MICROBIOLOGIA Year: 2019 Document type: Article Affiliation country: China Country of publication: United kingdom

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