The structure of a GH149 ß-(1 â 3) glucan phosphorylase reveals a new surface oligosaccharide binding site and additional domains that are absent in the disaccharide-specific GH94 glucose-ß-(1 â 3)-glucose (laminaribiose) phosphorylase.

Kuhaudomlarp, Sakonwan; Stevenson, Clare E M; Lawson, David M; Field, Robert A

The structure of a GH149 ß-(1 â 3) glucan phosphorylase reveals a new surface oligosaccharide binding site and additional domains that are absent in the disaccharide-specific GH94 glucose-ß-(1 â 3)-glucose (laminaribiose) phosphorylase.

Kuhaudomlarp, Sakonwan; Stevenson, Clare E M; Lawson, David M; Field, Robert A.

Affiliation

Kuhaudomlarp S; Department of Biological Chemistry, John Innes Centre, Norwich Research Park, Norwich, UK.
Stevenson CEM; Department of Biological Chemistry, John Innes Centre, Norwich Research Park, Norwich, UK.
Lawson DM; Department of Biological Chemistry, John Innes Centre, Norwich Research Park, Norwich, UK.
Field RA; Department of Biological Chemistry, John Innes Centre, Norwich Research Park, Norwich, UK.

Proteins ; 87(10): 885-892, 2019 10.

Article in En | MEDLINE | ID: mdl-31134667

Subject(s)

Bacterial Proteins/chemistry; Glucosyltransferases/chemistry; Glycosides/metabolism; Oligosaccharides/metabolism; Phosphorylases/chemistry; beta-Glucans/metabolism; Bacterial Proteins/metabolism; Binding Sites; Catalytic Domain; Crystallography, X-Ray; Glucosyltransferases/metabolism; Glycosides/chemistry; Models, Molecular; Oligosaccharides/chemistry; Phosphorylases/metabolism; Protein Conformation; Protein Domains; Substrate Specificity; beta-Glucans/chemistry

Key words

carbohydrate-active enzyme; glycoside phosphorylase; oligosaccharide; surface binding site

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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Oligosaccharides / Bacterial Proteins / Beta-Glucans / Glucosyltransferases / Phosphorylases / Glycosides Language: En Journal: Proteins Journal subject: BIOQUIMICA Year: 2019 Document type: Article Affiliation country: United kingdom Country of publication: United States

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