N-glycosylation and homodimeric folding significantly enhance the immunoreactivity of Mycobacterium tuberculosis virulence factor CFP32 when produced in the yeast Pichia pastoris.

Benabdessalem, Chaouki; Othman, Houcemeddine; Ouni, Rym; Ghouibi, Nabila; Dahman, Amira; Riahi, Rachid; Larguach, Beya; Srairi-Abid, Najet; Barbouche, Mohamed-Ridha; Fathallah, M Dahmani

Benabdessalem, Chaouki; Othman, Houcemeddine; Ouni, Rym; Ghouibi, Nabila; Dahman, Amira; Riahi, Rachid; Larguach, Beya; Srairi-Abid, Najet; Barbouche, Mohamed-Ridha; Fathallah, M Dahmani.

Affiliation

Benabdessalem C; Laboratory of Transmission, Control and Immunobiology of Infection - LR11IPT-02, Institut Pasteur de Tunis, Tunisia; University Tunis El Manar, Tunisia. Electronic address: chaouki.benabdessalem@pasteur.tn.
Othman H; Université de Tunis El Manar, Institut Pasteur de Tunis, LR16IPT08 Venins et Biomolécules Thérapeutiques, 1002, Tunis, Tunisia.
Ouni R; Laboratory of Transmission, Control and Immunobiology of Infection - LR11IPT-02, Institut Pasteur de Tunis, Tunisia; University of Carthage, Tunisia.
Ghouibi N; Laboratory of Transmission, Control and Immunobiology of Infection - LR11IPT-02, Institut Pasteur de Tunis, Tunisia; University Tunis El Manar, Tunisia.
Dahman A; Laboratory of Transmission, Control and Immunobiology of Infection - LR11IPT-02, Institut Pasteur de Tunis, Tunisia.
Riahi R; Laboratory of Transmission, Control and Immunobiology of Infection - LR11IPT-02, Institut Pasteur de Tunis, Tunisia.
Larguach B; Laboratory of Transmission, Control and Immunobiology of Infection - LR11IPT-02, Institut Pasteur de Tunis, Tunisia.
Jihene Bettaieb; Laboratory of Transmission, Control and Immunobiology of Infection - LR11IPT-02, Institut Pasteur de Tunis, Tunisia; University Tunis El Manar, Tunisia.
Srairi-Abid N; Université de Tunis El Manar, Institut Pasteur de Tunis, LR16IPT08 Venins et Biomolécules Thérapeutiques, 1002, Tunis, Tunisia; University Tunis El Manar, Tunisia.
Barbouche MR; Laboratory of Transmission, Control and Immunobiology of Infection - LR11IPT-02, Institut Pasteur de Tunis, Tunisia; University Tunis El Manar, Tunisia.
Fathallah MD; Laboratory of Transmission, Control and Immunobiology of Infection - LR11IPT-02, Institut Pasteur de Tunis, Tunisia.

Biochem Biophys Res Commun ; 516(3): 845-850, 2019 08 27.

Article in En | MEDLINE | ID: mdl-31262446

Subject(s)

Bacterial Proteins/chemistry; Epitopes/chemistry; Escherichia coli/genetics; Mycobacterium tuberculosis/genetics; Pichia/genetics; Protein Processing, Post-Translational; Amino Acid Sequence; Antibodies, Monoclonal/chemistry; Antibodies, Monoclonal/metabolism; Bacterial Proteins/genetics; Bacterial Proteins/immunology; Cloning, Molecular; Epitopes/genetics; Epitopes/immunology; Escherichia coli/metabolism; Gene Expression; Genetic Vectors/chemistry; Genetic Vectors/metabolism; Glycosylation; Models, Molecular; Mycobacterium bovis/genetics; Mycobacterium bovis/metabolism; Mycobacterium tuberculosis/immunology; Mycobacterium tuberculosis/metabolism; Mycobacterium tuberculosis/pathogenicity; Pichia/metabolism; Protein Conformation, alpha-Helical; Protein Conformation, beta-Strand; Protein Folding; Protein Interaction Domains and Motifs; Protein Multimerization; Recombinant Proteins/chemistry; Recombinant Proteins/genetics; Recombinant Proteins/immunology; Sequence Alignment; Sequence Homology, Amino Acid; Virulence Factors

Key words

CFP32; Immunogenicity; N-glycosylation; Pichia pastoris

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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Pichia / Bacterial Proteins / Protein Processing, Post-Translational / Escherichia coli / Mycobacterium tuberculosis / Epitopes Type of study: Prognostic_studies Language: En Journal: Biochem Biophys Res Commun Year: 2019 Document type: Article

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