Biochemical responses and proximate analysis of Piaractus brachypomus (Pisces: Characidae) exposed to phenanthrene.
Comp Biochem Physiol C Toxicol Pharmacol
; 228: 108649, 2020 Feb.
Article
in En
| MEDLINE
| ID: mdl-31669663
Polycyclic Aromatic Hydrocarbons (PAHs) are complex compounds generated from industrial and anthropogenic activities, although natural phenomena are also responsible for their presence in the environment. The aim of this study was to evaluate the effect of phenanthrene on proximate composition and antioxidant activity in Piaractus brachypomus during a subacute exposure. Fish were exposed intraperitoneally to phenanthrene (0.1, 1.0, and 10⯵gâ¯g-1) and a solvent control (0⯵gâ¯g-1). Muscle tissue, liver and bile were collected at 0â¯h, 11â¯days and 21â¯days. In dorsal muscle, parameters such as dry matter, protein, crude fat, and pH did not show alterations with exposure to phenanthrene (pâ¯≥â¯0.05). At 11â¯days, a significant increase of hepatic lipid peroxidation was observed in fish exposed to 10⯵gâ¯g-1. Additionally, a dose-dependent response was observed in the muscle, although no significant differences were observed in the activity of catalase and lipid peroxidation. Phenanthrene metabolites in bile were analyzed by fixed fluorescence at 260/380â¯nm (excitation/emission) wavelengths and levels indicated a dose-dependent response. Likewise, bioaccumulation of phenanthrene in bile was observed for 21â¯days, suggesting slow metabolism of this xenobiotic at 10⯵gâ¯g-1. This study provides important information on the oxidative effects generated by phenanthrene exposure for 21â¯days in P. brachypomus, generating fundamental information on adverse effects induced by PAHs on the most important Colombian native fish species. These data contribute to the development of additional research in environments contaminated with PAHs and can be applied to monitor areas contaminated with oil spills.
Key words
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Phenanthrenes
/
Water Pollutants, Chemical
/
Lipid Peroxidation
/
Xenobiotics
/
Characidae
Limits:
Animals
Language:
En
Journal:
Comp Biochem Physiol C Toxicol Pharmacol
Journal subject:
FARMACOLOGIA
/
TOXICOLOGIA
Year:
2020
Document type:
Article
Affiliation country:
Colombia
Country of publication:
United States