An Insight into Reprogramming Barriers to iPSC Generation.
Stem Cell Rev Rep
; 16(1): 56-81, 2020 02.
Article
in En
| MEDLINE
| ID: mdl-31758374
ABSTRACT
Derivation of induced Pluripotent Stem Cells (iPSCs) by reprogramming somatic cells to a pluripotent state has revolutionized stem cell research. Ensuing this, various groups have used genetic and non-genetic approaches to generate iPSCs from numerous cell types. However, achieving a pluripotent state in most of the reprogramming studies is marred by serious limitations such as low reprogramming efficiency and slow kinetics. These limitations are mainly due to the presence of potent barriers that exist during reprogramming when a mature cell is coaxed to achieve a pluripotent state. Several studies have revealed that intrinsic factors such as non-optimal stoichiometry of reprogramming factors, specific signaling pathways, cellular senescence, pluripotency-inhibiting transcription factors and microRNAs act as a roadblock. In addition, the epigenetic state of somatic cells and specific epigenetic modifications that occur during reprogramming also remarkably impede the generation of iPSCs. In this review, we present a comprehensive overview of the barriers that inhibit reprogramming and the understanding of which will pave the way to develop safe strategies for efficient reprogramming.
Key words
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Cell Lineage
/
MicroRNAs
/
Cellular Reprogramming
/
Induced Pluripotent Stem Cells
Limits:
Humans
Language:
En
Journal:
Stem Cell Rev Rep
Year:
2020
Document type:
Article
Affiliation country:
India