High yield production of the latex clearing protein from Gordonia polyisoprenivorans VH2 in fed batch fermentations using a recombinant strain of Escherichia coli.

Altenhoff, Anna-Lena; Thierbach, Sven; Steinbüchel, Alexander

Altenhoff, Anna-Lena; Thierbach, Sven; Steinbüchel, Alexander.

Affiliation

Altenhoff AL; Institut für Molekulare Mikrobiologie und Biotechnologie, Westfälische Wilhelms-Universität Münster, D-48149 Münster, Germany.
Thierbach S; Institut für Molekulare Mikrobiologie und Biotechnologie, Westfälische Wilhelms-Universität Münster, D-48149 Münster, Germany.
Steinbüchel A; Institut für Molekulare Mikrobiologie und Biotechnologie, Westfälische Wilhelms-Universität Münster, D-48149 Münster, Germany; Environmental Sciences Department, King Abdulaziz University, Jeddah, Saudi Arabia. Electronic address: steinbu@uni-muenster.de.

J Biotechnol ; 309: 92-99, 2020 Feb 10.

Article in En | MEDLINE | ID: mdl-31881242

Subject(s)

Actinobacteria/genetics; Actinobacteria/metabolism; Batch Cell Culture Techniques/methods; Escherichia coli/metabolism; Fermentation; Latex/metabolism; Recombinant Proteins/metabolism; Actinobacteria/growth & development; Bacterial Proteins/genetics; Bacterial Proteins/metabolism; Biomass; Culture Media/chemistry; Escherichia coli/genetics; Recombinant Proteins/genetics; Rubber

Key words

Escherichia coli; Fed batch fermentation; Latex clearing protein; Process development; Recombinant protein production

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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Recombinant Proteins / Actinobacteria / Escherichia coli / Fermentation / Batch Cell Culture Techniques / Latex Language: En Journal: J Biotechnol Journal subject: BIOTECNOLOGIA Year: 2020 Document type: Article Affiliation country: Germany Country of publication: Netherlands

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