Systematic optimization of square-wave electroporation conditions for bovine primary fibroblasts.

Hyder, Iqbal; Eghbalsaied, Shahin; Kues, Wilfried A

Hyder, Iqbal; Eghbalsaied, Shahin; Kues, Wilfried A.

Affiliation

Hyder I; Institute of Farm Animal Genetics, Friedrich-Loeffler-Institute, 31535, Neustadt, Germany.
Eghbalsaied S; Department of Veterinary Physiology, NTR College of Veterinary Science, Gannavaram, India.
Kues WA; Institute of Farm Animal Genetics, Friedrich-Loeffler-Institute, 31535, Neustadt, Germany.

BMC Mol Cell Biol ; 21(1): 9, 2020 Feb 28.

Article in En | MEDLINE | ID: mdl-32111153

Subject(s)

Electroporation/methods; Gene Transfer Techniques; Animals; Animals, Genetically Modified; Cattle; Cell Survival; Cells, Cultured; Fibroblasts/metabolism; Plasmids; Transfection

Key words

Electroporation; Fibroblasts; Square wave pulse; Transfection efficiency

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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Gene Transfer Techniques / Electroporation Limits: Animals Language: En Journal: BMC Mol Cell Biol Year: 2020 Document type: Article Affiliation country: Germany

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