Expression, Purification, and Applications of the Recombinant Lectin PVL from Psathyrella velutina Specific for Terminal N-Acetyl-Glucosamine.

Machon, Oriane; Varrot, Annabelle

Machon, Oriane; Varrot, Annabelle.

Affiliation

Machon O; Univ. Grenoble Alpes, CNRS, CERMAV, Grenoble, France.
Varrot A; Univ. Grenoble Alpes, CNRS, CERMAV, Grenoble, France. annabelle.varrot@cermav.cnrs.fr.

Methods Mol Biol ; 2132: 421-436, 2020.

Article in En | MEDLINE | ID: mdl-32306349

Subject(s)

Acetylglucosamine/analysis; Agaricales/metabolism; Lectins/metabolism; Acetylglucosamine/metabolism; Agaricales/genetics; Binding Sites; Escherichia coli/genetics; Escherichia coli/growth & development; Fungal Proteins/genetics; Fungal Proteins/metabolism; Lectins/chemistry; Lectins/genetics; Models, Molecular; Protein Conformation; Protein Engineering; Recombinant Proteins/chemistry; Recombinant Proteins/metabolism

Key words

ELLA; Lectin probe; O-GlcNAcylation detection; Terminal O-GlcNAc; Tumor markers

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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Acetylglucosamine / Agaricales / Lectins Language: En Journal: Methods Mol Biol Journal subject: BIOLOGIA MOLECULAR Year: 2020 Document type: Article Affiliation country: France Country of publication: United States

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