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Development and validation of an UHPLC-MS/MS method for extended serum steroid profiling in female populations.
Salamin, Olivier; Ponzetto, Federico; Cauderay, Michel; Boccard, Julien; Rudaz, Serge; Saugy, Martial; Kuuranne, Tiia; Nicoli, Raul.
Affiliation
  • Salamin O; Center of Research & Expertise in Anti-Doping sciences, REDs, University of Lausanne, Switzerland.
  • Ponzetto F; Swiss Laboratory for Doping Analyses, University Center of Legal Medicine, Lausanne & Geneva, Lausanne University Hospital & University of Lausanne, Switzerland.
  • Cauderay M; Center of Research & Expertise in Anti-Doping sciences, REDs, University of Lausanne, Switzerland.
  • Boccard J; Swiss Laboratory for Doping Analyses, University Center of Legal Medicine, Lausanne & Geneva, Lausanne University Hospital & University of Lausanne, Switzerland.
  • Rudaz S; Fondation USCADE, Pully, Switzerland.
  • Saugy M; Institute of Pharmaceutical Sciences of Western Switzerland, University of Geneva, CMU-Rue Michel Servet 1, 1211 Geneva 4, Switzerland.
  • Kuuranne T; Institute of Pharmaceutical Sciences of Western Switzerland, University of Geneva, CMU-Rue Michel Servet 1, 1211 Geneva 4, Switzerland.
  • Nicoli R; Center of Research & Expertise in Anti-Doping sciences, REDs, University of Lausanne, Switzerland.
Bioanalysis ; 12(11): 753-768, 2020 Jun.
Article in En | MEDLINE | ID: mdl-32479744
Aim: Quantitative endogenous steroid profiling in blood appears as a complementary approach to the urinary module of the World Anti-Doping Agency's Athlete Biological Passport Steroidal Module for the detection of testosterone doping. To refine this approach further, a UHPLC-MS/MS method was developed for the simultaneous determination of 14 free and 14 conjugated steroids in serum. Results: The method was validated for quantitative purposes with satisfactory results in terms of selectivity, linearity range, trueness, precision and combined uncertainty (<20%). The validated method was then applied to serum samples from both healthy women and women diagnosed with mild hyperandrogenism. Conclusion: The UHPLC-MS/MS method showed promising capability in quantifying free and conjugated steroids in serum and determining variations of their concentration/distribution within serum samples from different populations.
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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Steroids / Substance Abuse Detection / Doping in Sports Limits: Female / Humans Language: En Journal: Bioanalysis Year: 2020 Document type: Article Affiliation country: Switzerland Country of publication: United kingdom
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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Steroids / Substance Abuse Detection / Doping in Sports Limits: Female / Humans Language: En Journal: Bioanalysis Year: 2020 Document type: Article Affiliation country: Switzerland Country of publication: United kingdom