Expression, purification and crystal structure determination of a ferredoxin reductase from the actinobacterium Thermobifida fusca.

Rodriguez Buitrago, Jhon Alexander; Klünemann, Thomas; Blankenfeldt, Wulf; Schallmey, Anett

Rodriguez Buitrago, Jhon Alexander; Klünemann, Thomas; Blankenfeldt, Wulf; Schallmey, Anett.

Affiliation

Rodriguez Buitrago JA; Institute for Biochemistry, Biotechnology and Bioinformatics, Technical University Braunschweig, Spielmannstrasse 7, 38106 Braunschweig, Germany.
Klünemann T; Structure and Function of Proteins, Helmholtz Centre for Infection Research, Inhoffenstrasse 7, 38124 Braunschweig, Germany.
Blankenfeldt W; Institute for Biochemistry, Biotechnology and Bioinformatics, Technical University Braunschweig, Spielmannstrasse 7, 38106 Braunschweig, Germany.
Schallmey A; Institute for Biochemistry, Biotechnology and Bioinformatics, Technical University Braunschweig, Spielmannstrasse 7, 38106 Braunschweig, Germany.

Acta Crystallogr F Struct Biol Commun ; 76(Pt 8): 334-340, 2020 Aug 01.

Article in En | MEDLINE | ID: mdl-32744244

Subject(s)

Bacterial Proteins/chemistry; Coenzymes/chemistry; Ferredoxins/chemistry; NAD/chemistry; Oxidoreductases/chemistry; Amino Acid Sequence; Bacterial Proteins/genetics; Bacterial Proteins/metabolism; Binding Sites; Cloning, Molecular; Coenzymes/metabolism; Crystallography, X-Ray; Escherichia coli/genetics; Escherichia coli/metabolism; Ferredoxins/genetics; Ferredoxins/metabolism; Flavin-Adenine Dinucleotide/chemistry; Flavin-Adenine Dinucleotide/metabolism; Gene Expression; Genetic Vectors/chemistry; Genetic Vectors/metabolism; Models, Molecular; NAD/metabolism; Oxidoreductases/genetics; Oxidoreductases/metabolism; Protein Binding; Protein Conformation, alpha-Helical; Protein Conformation, beta-Strand; Protein Interaction Domains and Motifs; Recombinant Proteins/chemistry; Recombinant Proteins/genetics; Recombinant Proteins/metabolism; Sequence Alignment; Sequence Homology, Amino Acid; Thermobifida/chemistry; Thermobifida/enzymology

Key words

Thermobifida fusca; cytochrome P450; ferredoxin reductase

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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Oxidoreductases / Bacterial Proteins / Coenzymes / Ferredoxins / NAD Language: En Journal: Acta Crystallogr F Struct Biol Commun Year: 2020 Document type: Article Affiliation country: Germany Country of publication: United States

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