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Influence of organic solvents in the extraction and purification of torularhodin from Sporobolomyces ruberrimus.
Kanno, Karen Yuri Feitosa; Karp, Susan Grace; Rodrigues, Cristine; de Andrade Tanobe, Valcineide Oliveira; Soccol, Carlos Ricardo; da Costa Cardoso, Ligia Alves.
Affiliation
  • Kanno KYF; Doctorate and Master's Degree Graduation Program in Industrial Biotechnology, Universidade Positivo, Curitiba, PR, Brazil.
  • Karp SG; Graduate Program in Bioprocess Engineering and Biotechnology, Universidade Federal do Paraná, Curitiba, PR, Brazil.
  • Rodrigues C; Graduate Program in Bioprocess Engineering and Biotechnology, Universidade Federal do Paraná, Curitiba, PR, Brazil.
  • de Andrade Tanobe VO; Graduate Program in Bioprocess Engineering and Biotechnology, Universidade Federal do Paraná, Curitiba, PR, Brazil.
  • Soccol CR; Graduate Program in Bioprocess Engineering and Biotechnology, Universidade Federal do Paraná, Curitiba, PR, Brazil.
  • da Costa Cardoso LA; Doctorate and Master's Degree Graduation Program in Industrial Biotechnology, Universidade Positivo, Curitiba, PR, Brazil. ligiacardoso@up.edu.br.
Biotechnol Lett ; 43(1): 89-98, 2021 Jan.
Article in En | MEDLINE | ID: mdl-33064227
OBJECTIVE: This work aimed at evaluating the influence of organic solvents and stationary phases in the extraction with glass beads and chromatographic purification of carotenoids, especially torularhodin, from Sporobolomyces ruberrimus. RESULTS: The combinations of acetone:hexane (1:1 v/v) and acetone:ethyl ether (1:1 v/v) yielded 171.74 and 172.19 µg of total carotenoids.g of cells-1, respectively. The first blend resulted in the highest percent of cell lysis of 57.4%. Among different proportions of acetone:hexane, the 9:1 v/v mixture showed a significant difference (p < 0.05), resulting in a recovery of total carotenoids of 221.88 µg.g of cells-1. The purification of carotenoids was made by preparative chromatography and the yield of the silica-containing stationary phase was higher (24 µg torularhodin.g cells-1). The analyses of the purified fractions in thin layer chromatography and high performance liquid chromatography indicated that the purification of carotenoids, especially of torularhodin, was successfully performed. CONCLUSIONS: The combination of polar (acetone) and non-polar solvents (hexane) and the use of silica as stationary phase was efficient to recover and purify torularhodin from the intracellular pigments of Sporobolomyces ruberrimus.
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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Solvents / Basidiomycota / Carotenoids Language: En Journal: Biotechnol Lett Year: 2021 Document type: Article Affiliation country: Brazil Country of publication: Netherlands

Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Solvents / Basidiomycota / Carotenoids Language: En Journal: Biotechnol Lett Year: 2021 Document type: Article Affiliation country: Brazil Country of publication: Netherlands