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Geographic distribution of sex chromosome polymorphism in Anastrepha fraterculus sp. 1 from Argentina.
Giardini, María Cecilia; Nieves, Mariela; Scannapieco, Alejandra Carla; Conte, Claudia Alejandra; Milla, Fabián Horacio; Schapovaloff, María Elena; Frissolo, Maria Soledad; Remis, María Isabel; Cladera, Jorge Luis; Lanzavecchia, Silvia Beatriz.
Affiliation
  • Giardini MC; Laboratorio de Insectos de Importancia Agronómica, Instituto de Genética (IGEAF), Instituto de Agrobiotecnología y Biología Molecular (IABIMO), INTA- CONICET, Hurlingham, Buenos Aires, Argentina.
  • Nieves M; Grupo de Investigación en Biología Evolutiva, Departamento de Ecología, Genética y Evolución, IEGEBA (CONICET), Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Buenos Aires, Argentina.
  • Scannapieco AC; Laboratorio de Insectos de Importancia Agronómica, Instituto de Genética (IGEAF), Instituto de Agrobiotecnología y Biología Molecular (IABIMO), INTA- CONICET, Hurlingham, Buenos Aires, Argentina.
  • Conte CA; Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Buenos Aires, Argentina.
  • Milla FH; Laboratorio de Insectos de Importancia Agronómica, Instituto de Genética (IGEAF), Instituto de Agrobiotecnología y Biología Molecular (IABIMO), INTA- CONICET, Hurlingham, Buenos Aires, Argentina.
  • Schapovaloff ME; Laboratorio de Insectos de Importancia Agronómica, Instituto de Genética (IGEAF), Instituto de Agrobiotecnología y Biología Molecular (IABIMO), INTA- CONICET, Hurlingham, Buenos Aires, Argentina.
  • Frissolo MS; Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Buenos Aires, Argentina.
  • Remis MI; Estación Experimental Agropecuaria Montecarlo, Instituto Nacional de Tecnología Agropecuaria (INTA), Misiones, Argentina.
  • Cladera JL; Subprograma La Rioja, Programa Nacional de Control y Erradicación de Moscas de los Frutos (PROCEM), La Rioja, Argentina.
  • Lanzavecchia SB; Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Buenos Aires, Argentina.
BMC Genet ; 21(Suppl 2): 149, 2020 12 18.
Article in En | MEDLINE | ID: mdl-33339514
BACKGROUND: Anastrepha fraterculus is recognized as a quarantine pest in several American countries. This fruit fly species is native to the American continent and distributed throughout tropical and subtropical regions. It has been reported as a complex of cryptic species, and at least eight morphotypes have been described. Only one entity of this complex, formerly named Anastrepha fraterculus sp. 1, is present in Argentina. Previous cytogenetic studies on this morphotype described the presence of sex chromosome variation identified by chromosomal size and staining patterns. In this work, we expanded the cytological study of this morphotype by analyzing laboratory strains and wild populations to provide information about the frequency and geographic distribution of these sex chromosome variants. We analyzed the mitotic metaphases of individuals from four laboratory strains and five wild populations from the main fruit-producing areas of Argentina, including the northwest (Tucumán and La Rioja), northeast (Entre Ríos and Misiones), and center (Buenos Aires) of the country. RESULTS: In wild samples, we observed a high frequency of X1X1 (0.94) and X1Y5 (0.93) karyomorphs, whereas X1X2 and X1Y6 were exclusively found at a low frequency in Buenos Aires (0.07 and 0.13, respectively), Entre Ríos (0.16 and 0.14, respectively) and Tucumán (0.03 and 0.04, respectively). X2X2 and X2Y5 karyomorphs were not found in wild populations but were detected at a low frequency in laboratory strains. In fact, karyomorph frequencies differed between wild populations and laboratory strains. No significant differences among A. fraterculus wild populations were evidenced in either karyotypic or chromosomal frequencies. However, a significant correlation was observed between Y5 chromosomal frequency and latitude. CONCLUSIONS: We discuss the importance of cytogenetics to understand the possible route of invasion and dispersion of this pest in Argentina and the evolutionary forces acting under laboratory conditions, possibly driving changes in the chromosomal frequencies. Our findings provide deep and integral genetic knowledge of this species, which has become of relevance to the characterization and selection of valuable A. fraterculus sp. 1 strains for mass rearing production and SIT implementation.
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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Polymorphism, Genetic / Sex Chromosomes / Tephritidae / Chromosomes, Insect / Genetics, Population Type of study: Prognostic_studies Limits: Animals Country/Region as subject: America do sul / Argentina Language: En Journal: BMC Genet Journal subject: BIOLOGIA MOLECULAR / BIOTECNOLOGIA Year: 2020 Document type: Article Affiliation country: Argentina Country of publication: United kingdom

Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Polymorphism, Genetic / Sex Chromosomes / Tephritidae / Chromosomes, Insect / Genetics, Population Type of study: Prognostic_studies Limits: Animals Country/Region as subject: America do sul / Argentina Language: En Journal: BMC Genet Journal subject: BIOLOGIA MOLECULAR / BIOTECNOLOGIA Year: 2020 Document type: Article Affiliation country: Argentina Country of publication: United kingdom