Thermophilic anaerobic digestion of winery effluents in a two-stage process and the effect of the feeding frequency on methane production.

This investigation evaluates a two-stage process to treat highly concentrated winery effluents, including a thermophilic methanogenic stage. The inoculum adaptation, the effect of the organic loading rates on both stages, and the methanogenic reactor's feeding frequency on the process performance were studied. An active thermophilic inoculum was obtained by a one-step temperature increase from 35 to 55 °C. The application of organic loads above 120 kg COD m-3 d-1 in the acidogenic stage ensured the highest acetic acid concentration, while methane production rates as high as 7.1 Nm3 CH4 m-3 d-1 and a yield of 348 L CH4 kg-1 COD were obtained in the thermophilic methanogenic stage using an organic loading rate of 29.9 kg COD m-3 d-1. However, a lower removal of organic matter was observed under that condition. Lower feeding frequencies improved methane productivity and yield, suggesting that this parameter is a useful process optimization tool.