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Acellular dermal matrix in skin wound healing in rabbits - histological and histomorphometric analyses.
Carvalho-Júnior, José da Conceição; Zanata, Fabiana; Aloise, Antônio Carlos; Ferreira, Lydia Masako.
Affiliation
  • Carvalho-Júnior JDC; Divisao de Cirurgia Plastica e Reconstrutiva, Universidade Federal de Sao Paulo, Sao Paulo, SP, BR.
  • Zanata F; Divisao de Cirurgia Plastica e Reconstrutiva, Universidade Federal de Sao Paulo, Sao Paulo, SP, BR.
  • Aloise AC; Divisao de Cirurgia Plastica e Reconstrutiva, Universidade Federal de Sao Paulo, Sao Paulo, SP, BR.
  • Ferreira LM; Divisao de Cirurgia Plastica e Reconstrutiva, Universidade Federal de Sao Paulo, Sao Paulo, SP, BR.
Clinics (Sao Paulo) ; 76: e2066, 2021.
Article in En | MEDLINE | ID: mdl-33681941
OBJECTIVES: To analyze the histology and histomorphometry of healing associated with acellular dermal matrix in skin wounds in rabbits. METHODS: Twelve male rabbits were divided into two groups: the control group (CG) and the matrix group (MG). Three skin wounds with a total area of 20 × 20 mm were created on the dorsal region of each animal. Photographic records of the lesions taken over a 21-day period and use of the ImageJ program allowed calculation of the wound contraction rate. The lesions were biopsied on days 3, 14 and 21 for histomorphometric analysis to define the thicknesses of the dermis and epidermis (hematoxylin-eosin) and calculate the densities of type I and type III collagen (picrosirius). RESULTS: No significant difference in the healing rate was found between the groups (p>0.05). The MG presented greater epidermal thickness on day 3 (p<0.05) and on days 14 and 21 (p<0.001). The MG presented greater dermal thickness throughout the study period (p<0.05). The type I collagen density was higher in the MG throughout the study period (p<0.05), and the type III collagen density was higher in the MG on days 3 and 14 (p<0.05) and on day 21 (p<0.001). CONCLUSION: The use of acellular dermal matrix increased the thickness of the dermal and epidermal layers and the amount of type I and III collagen during skin wound healing and did not alter the rate of wound contraction.
Subject(s)

Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Acellular Dermis Limits: Animals Language: En Journal: Clinics (Sao Paulo) Journal subject: MEDICINA Year: 2021 Document type: Article Country of publication: United States

Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Acellular Dermis Limits: Animals Language: En Journal: Clinics (Sao Paulo) Journal subject: MEDICINA Year: 2021 Document type: Article Country of publication: United States