In vitro production and multiplex quantification of 2',3'-cyclic phosphate-containing 5'-tRNA half molecules.

Kawamura, Takuya; Shigematsu, Megumi; Kirino, Yohei

Kawamura, Takuya; Shigematsu, Megumi; Kirino, Yohei.

Affiliation

Kawamura T; Computational Medicine Center, Sidney Kimmel Medical College, Thomas Jefferson University, Philadelphia, PA, USA.
Shigematsu M; Computational Medicine Center, Sidney Kimmel Medical College, Thomas Jefferson University, Philadelphia, PA, USA.
Kirino Y; Computational Medicine Center, Sidney Kimmel Medical College, Thomas Jefferson University, Philadelphia, PA, USA. Electronic address: Yohei.Kirino@jefferson.edu.

Methods ; 203: 335-341, 2022 07.

Article in En | MEDLINE | ID: mdl-33962012

Subject(s)

Phosphates; RNA, Transfer; Humans; RNA/genetics; RNA, Transfer/genetics; RNA, Untranslated; Sequence Analysis, RNA/methods

Key words

2',3'-cyclic phosphate (cP); 5'-tRNA half; Multiplex; Plasma; TaqMan RT-qPCR; cP-RNA

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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Phosphates / RNA, Transfer Limits: Humans Language: En Journal: Methods Journal subject: BIOQUIMICA Year: 2022 Document type: Article Affiliation country: United States Country of publication: United States

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