Optimisation of sample storage and DNA extraction for human gut microbiota studiesâ.

Kazantseva, Jekaterina; Malv, Esther; Kaleda, Aleksei; Kallastu, Aili; Meikas, Anne

Optimisation of sample storage and DNA extraction for human gut microbiota studiesâ.

Kazantseva, Jekaterina; Malv, Esther; Kaleda, Aleksei; Kallastu, Aili; Meikas, Anne.

Affiliation

Kazantseva J; Center of Food and Fermentation Technologies, Akadeemia tee 15a, 12618, Tallinn, Estonia. jekaterina@tftak.eu.
Malv E; Center of Food and Fermentation Technologies, Akadeemia tee 15a, 12618, Tallinn, Estonia.
Kaleda A; Center of Food and Fermentation Technologies, Akadeemia tee 15a, 12618, Tallinn, Estonia.
Kallastu A; Center of Food and Fermentation Technologies, Akadeemia tee 15a, 12618, Tallinn, Estonia.
Meikas A; Center of Food and Fermentation Technologies, Akadeemia tee 15a, 12618, Tallinn, Estonia.

BMC Microbiol ; 21(1): 158, 2021 05 29.

Article in En | MEDLINE | ID: mdl-34051731

Subject(s)

Bacteria/genetics; DNA, Bacterial/isolation & purification; Gastrointestinal Microbiome; Metagenomics/methods; Preservation, Biological/methods; Bacteria/classification; Bacteria/isolation & purification; DNA, Bacterial/genetics; Feces/microbiology; High-Throughput Nucleotide Sequencing; Humans; RNA, Ribosomal, 16S/genetics

Key words

16S rRNA gene sequencing; Faecal DNA extraction; Faecal sample storage; Human gut microbiota; Illumina iSeq 100, DNA/RNA Shield solution

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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Preservation, Biological / Bacteria / DNA, Bacterial / Metagenomics / Gastrointestinal Microbiome Type of study: Evaluation_studies Limits: Humans Language: En Journal: BMC Microbiol Journal subject: MICROBIOLOGIA Year: 2021 Document type: Article Affiliation country: Estonia Country of publication: United kingdom

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