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Modification of a spectrophotometric method for assessment of monoamine oxidase activity with 2,4-dinitrophenylhydrazine as a derivatizing reagent.
Vinel, Polina K; Grobovoy, S I; Sinitskii, A I; Kolesnikov, O L.
Affiliation
  • Vinel PK; South Ural State Medical University, 64 Vorovskogo st., 454092, Chelyabinsk, Russia. Electronic address: vinelpolina@icloud.com.
  • Grobovoy SI; South Ural State Medical University, 64 Vorovskogo st., 454092, Chelyabinsk, Russia.
  • Sinitskii AI; South Ural State Medical University, 64 Vorovskogo st., 454092, Chelyabinsk, Russia.
  • Kolesnikov OL; South Ural State Medical University, 64 Vorovskogo st., 454092, Chelyabinsk, Russia.
Anal Biochem ; 629: 114294, 2021 09 15.
Article in En | MEDLINE | ID: mdl-34197779
The aim of the study was to modify a simple and widely used spectrophotometric assay for MAO activity evaluation with 2,4-dinitrophenylhydrazine. A modified procedure includes molar absorption coefficients of 2,4-DNP-hydrazone benzaldehyde and 2,4-DNP-hydrazone 5-hydroxyindolylacetaldehyde as 2.3 × 104mol-1l cm-1 and 1.0 × 104 mol-1l cm-1, respectively. Such an approach allows to express specific enzyme activity as nmol product formed/min/mg protein.
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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Phenylhydrazines / Monoamine Oxidase / Monoamine Oxidase Inhibitors Language: En Journal: Anal Biochem Year: 2021 Document type: Article Country of publication: United States
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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Phenylhydrazines / Monoamine Oxidase / Monoamine Oxidase Inhibitors Language: En Journal: Anal Biochem Year: 2021 Document type: Article Country of publication: United States