Knock-in tagging in zebrafish facilitated by insertion into non-coding regions.

Levic, Daniel S; Yamaguchi, Naoya; Wang, Siyao; Knaut, Holger; Bagnat, Michel

Levic, Daniel S; Yamaguchi, Naoya; Wang, Siyao; Knaut, Holger; Bagnat, Michel.

Affiliation

Levic DS; Department of Cell Biology, Duke University, Durham, NC 27710, USA.
Yamaguchi N; Skirball Institute of Biomolecular Medicine, New York University Grossman School of Medicine, Department of Cell Biology, New York, NY 10016, USA.
Wang S; Department of Cell Biology, Duke University, Durham, NC 27710, USA.
Knaut H; Skirball Institute of Biomolecular Medicine, New York University Grossman School of Medicine, Department of Cell Biology, New York, NY 10016, USA.
Bagnat M; Department of Cell Biology, Duke University, Durham, NC 27710, USA.

Development ; 148(19)2021 10 01.

Article in En | MEDLINE | ID: mdl-34495314

Subject(s)

Gene Knock-In Techniques/methods; Green Fluorescent Proteins/genetics; Zebrafish Proteins/genetics; Animals; Green Fluorescent Proteins/metabolism; Mutagenesis, Insertional; Recombinant Proteins/genetics; Recombinant Proteins/metabolism; Regulatory Sequences, Nucleic Acid/genetics; Zebrafish; Zebrafish Proteins/metabolism

Key words

CRISPR; Epithelial; Knock-in; Morphogenesis; Quantitative imaging; Zebrafish

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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Zebrafish Proteins / Green Fluorescent Proteins / Gene Knock-In Techniques Limits: Animals Language: En Journal: Development Journal subject: BIOLOGIA / EMBRIOLOGIA Year: 2021 Document type: Article Affiliation country: United States Country of publication: United kingdom

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