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Toxicity of spike fragments SARS-CoV-2 S protein for zebrafish: A tool to study its hazardous for human health?
Ventura Fernandes, Bianca H; Feitosa, Natália Martins; Barbosa, Ana Paula; Bomfim, Camila Gasque; Garnique, Anali M B; Rosa, Ivana F; Rodrigues, Maira S; Doretto, Lucas B; Costa, Daniel F; Camargo-Dos-Santos, Bruno; Franco, Gabrielli A; Neto, João Favero; Lunardi, Juliana Sartori; Bellot, Marina Sanson; Alves, Nina Pacheco Capelini; Costa, Camila C; Aracati, Mayumi F; Rodrigues, Letícia F; Costa, Camila C; Cirilo, Rafaela Hemily; Colagrande, Raul Marcelino; Gomes, Francisco I F; Nakajima, Rafael T; Belo, Marco A A; Giaquinto, Percília Cardoso; de Oliveira, Susana Luporini; Eto, Silas Fernandes; Fernandes, Dayanne Carla; Manrique, Wilson G; Conde, Gabriel; Rosales, Roberta R C; Todeschini, Iris; Rivero, Ilo; Llontop, Edgar; Sgro, Germán G; Oka, Gabriel Umaji; Bueno, Natalia Fernanda; Ferraris, Fausto K; de Magalhães, Mariana T Q; Medeiros, Renata J; Mendonça-Gomes, Juliana M; Junqueira, Mara Souza; Conceição, Kátia; Pontes, Leticia Gomes de; Condino-Neto, Antonio; Perez, Andrea C; Barcellos, Leonardo J G; Júnior, José Dias Correa; Dorlass, Erick Gustavo; Camara, Niels O S.
Affiliation
  • Ventura Fernandes BH; Laboratório de Controle Genético e Sanitário, Diretoria Técnica de Apoio ao Ensino e Pesquisa, Faculdade de Medicina da Universidade de São Paulo, Brazil.
  • Feitosa NM; Laboratório Integrado de Biociências Translacionais (LIBT), Instituto de Biodiversidade e Sustentabilidade (NUPEM), Universidade Federal do Rio de Janeiro (UFRJ), Macaé, RJ, Brazil.
  • Barbosa AP; Department of Microbiology, Institute of Biomedical Sciences, University of Sao Paulo, Sao Paulo, Brazil.
  • Bomfim CG; Department of Microbiology, Institute of Biomedical Sciences, University of Sao Paulo, Sao Paulo, Brazil.
  • Garnique AMB; Department of Cell Biology, Institute of Biomedical Sciences, University of São Paulo, Brazil.
  • Rosa IF; Reproductive and Molecular Biology Group, Department of Morphology, Institute of Biosciences, Sao Paulo State University, Botucatu, São Paulo, Brazil.
  • Rodrigues MS; Reproductive and Molecular Biology Group, Department of Morphology, Institute of Biosciences, Sao Paulo State University, Botucatu, São Paulo, Brazil.
  • Doretto LB; Reproductive and Molecular Biology Group, Department of Morphology, Institute of Biosciences, Sao Paulo State University, Botucatu, São Paulo, Brazil.
  • Costa DF; Reproductive and Molecular Biology Group, Department of Morphology, Institute of Biosciences, Sao Paulo State University, Botucatu, São Paulo, Brazil.
  • Camargo-Dos-Santos B; Department of Structural and Functional Biology, Institute of Biosciences of Botucatu, São Paulo State University, SP, Brazil.
  • Franco GA; Department of Structural and Functional Biology, Institute of Biosciences of Botucatu, São Paulo State University, SP, Brazil.
  • Neto JF; Department of Structural and Functional Biology, Institute of Biosciences of Botucatu, São Paulo State University, SP, Brazil.
  • Lunardi JS; Department of Structural and Functional Biology, Institute of Biosciences of Botucatu, São Paulo State University, SP, Brazil.
  • Bellot MS; Department of Structural and Functional Biology, Institute of Biosciences of Botucatu, São Paulo State University, SP, Brazil.
  • Alves NPC; Department of Structural and Functional Biology, Institute of Biosciences of Botucatu, São Paulo State University, SP, Brazil.
  • Costa CC; Department of Preventive Veterinary Medicine, São Paulo State University (UNESP), Jaboticabal, Brazil.
  • Aracati MF; Department of Preventive Veterinary Medicine, São Paulo State University (UNESP), Jaboticabal, Brazil.
  • Rodrigues LF; Department of Preventive Veterinary Medicine, São Paulo State University (UNESP), Jaboticabal, Brazil.
  • Costa CC; Department of Preventive Veterinary Medicine, São Paulo State University (UNESP), Jaboticabal, Brazil.
  • Cirilo RH; Department of Structural and Functional Biology, Institute of Biosciences of Botucatu, São Paulo State University, SP, Brazil.
  • Colagrande RM; Department of Structural and Functional Biology, Institute of Biosciences of Botucatu, São Paulo State University, SP, Brazil.
  • Gomes FIF; Department of Pharmacology, Center of Research in Inflammatory Diseases, Ribeirao Preto Medical School, University of São Paulo, Brazil.
  • Nakajima RT; Reproductive and Molecular Biology Group, Department of Morphology, Institute of Biosciences, Sao Paulo State University, Botucatu, São Paulo, Brazil.
  • Belo MAA; Brasil University, Descalvado, São Paulo, Brazil.
  • Giaquinto PC; Universidade Estadual Paulista Júlio de Mesquita Filho, Instituto de Biociências - Departamento de Fisiologia, São Paulo, Brazil.
  • de Oliveira SL; Universidade Estadual Paulista Júlio de Mesquita Filho, São Paulo, Brazil.
  • Eto SF; Postgraduate Program in Health Sciences, PROCISA, Federal University of Roraima, Brazil.
  • Fernandes DC; Immunochemistry Laboratory, Butantan Institute, São Paulo, Brazil.
  • Manrique WG; Aquaculture Health Research and Extension Group, GRUPESA, Aquaculture Health Laboratory, LABSA, Department of Veterinary Medicine, Federal University of Rondônia, Rolim de Moura campus, Rondônia, Brazil.
  • Conde G; Department of Preventive Veterinary Medicine, São Paulo State University, Jaboticabal, Brazil.
  • Rosales RRC; Department of Cell and Molecular Biology, Ribeirao Preto Medical School, University of São Paulo, Ribeirão Preto, Brazil.
  • Todeschini I; Departamento de Bioquímica, Instituto de Química, Universidade de São Paulo, Brazil.
  • Rivero I; Pontifícia Universidade Católica de Minas Gerais, Brazil.
  • Llontop E; Departamento de Bioquímica, Instituto de Química, Universidade de São Paulo, Brazil.
  • Sgro GG; Departamento de Bioquímica, Instituto de Química, Universidade de São Paulo, Brazil; Departamento de Ciências Biomoleculares, Faculdade de Ciências Farmacêuticas de Ribeirão Preto, Universidade de São Paulo, Ribeirão Preto, SP, Brazil.
  • Oka GU; Departamento de Bioquímica, Instituto de Química, Universidade de São Paulo, Brazil.
  • Bueno NF; Departamento de Bioquímica, Instituto de Química, Universidade de São Paulo, Brazil.
  • Ferraris FK; Department of Pharmacology and Toxicology, Oswaldo Cruz Foundation, FIOCRUZ, Rio de Janeiro, Brazil.
  • de Magalhães MTQ; Department of Biochemistry and Immunology, Institute of Biological Sciences, Federal University of Minas Gerais, Belo Horizonte, Brazil.
  • Medeiros RJ; Laboratory of Physiology, INCQS/Fiocruz Zebrafish Facility, Departament of Pharmacology and Toxicology, National Institute for Quality Control in Health, Brasil.
  • Mendonça-Gomes JM; Transplantation Immunobiology Lab, Department of Immunology, Institute of Biomedical Sciences, Universidade de Sao Paulo, Brazil.
  • Junqueira MS; Center for Translational Research in Oncology, Cancer Institute of the State of Sao Paulo, Faculty of Medicine, University of São Paulo, Sao Paulo, Brazil.
  • Conceição K; Laboratory of Peptide Biochemistry, Federal University of São Paulo, Brazil.
  • Pontes LG; Laboratory of Human Immunology, Department Immunology, Institute Biomedical Sciences, University São Paulo, Sao Paulo, Brazil.
  • Condino-Neto A; Laboratory of Human Immunology, Department Immunology, Institute Biomedical Sciences, University São Paulo, Sao Paulo, Brazil.
  • Perez AC; Department of Pharmacology, Universidade Federal de Minas Gerais, Brazil.
  • Barcellos LJG; Graduate Program of Pharmacology, Federal University of Santa Maria, Brazil; Laboratory of Fish Physiology, Graduate Program of Bioexperimentation and of Environmental Sciences, University of Passo Fundo, Brazil.
  • Júnior JDC; Laboratório do Estudo da Interação Químico Biológica e da Reprodução Animal, LIQBRA, Bloco O3,174, Brazil; Departamento de Morfologia Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais, Brazil.
  • Dorlass EG; Department of Microbiology, Institute of Biomedical Sciences, University of Sao Paulo, Brazil.
  • Camara NOS; Transplantation Immunobiology Lab, Department of Immunology, Institute of Biomedical Sciences, Universidade de Sao Paulo, Brazil.
Sci Total Environ ; 813: 152345, 2022 Mar 20.
Article in En | MEDLINE | ID: mdl-34942250
Despite the significant increase in the generation of SARS-CoV-2 contaminated domestic and hospital wastewater, little is known about the ecotoxicological effects of the virus or its structural components in freshwater vertebrates. In this context, this study evaluated the deleterious effects caused by SARS-CoV-2 Spike protein on the health of Danio rerio, zebrafish. We demonstrated, for the first time, that zebrafish injected with fragment 16 to 165 (rSpike), which corresponds to the N-terminal portion of the protein, presented mortalities and adverse effects on liver, kidney, ovary and brain tissues. The conserved genetic homology between zebrafish and humans might be one of the reasons for the intense toxic effects followed inflammatory reaction from the immune system of zebrafish to rSpike which provoked damage to organs in a similar pattern as happen in severe cases of COVID-19 in humans, and, resulted in 78,6% of survival rate in female adults during the first seven days. The application of spike protein in zebrafish was highly toxic that is suitable for future studies to gather valuable information about ecotoxicological impacts, as well as vaccine responses and therapeutic approaches in human medicine. Therefore, besides representing an important tool to assess the harmful effects of SARS-CoV-2 in the aquatic environment, we present the zebrafish as an animal model for translational COVID-19 research.
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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Spike Glycoprotein, Coronavirus / COVID-19 Limits: Animals / Female / Humans Language: En Journal: Sci Total Environ Year: 2022 Document type: Article Affiliation country: Brazil Country of publication: Netherlands
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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Spike Glycoprotein, Coronavirus / COVID-19 Limits: Animals / Female / Humans Language: En Journal: Sci Total Environ Year: 2022 Document type: Article Affiliation country: Brazil Country of publication: Netherlands