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Mutagenesis analysis of GMN motif in Arabidopsis thaliana Mg2+ transporter MRS2-1.
Yang, Xiaoyu; Kobayashi, Natsuko I; Hayashi, Yoshiki; Ito, Koichi; Moriwaki, Yoshitaka; Terada, Tohru; Shimizu, Kentaro; Hattori, Motoyuki; Iwata, Ren; Suzuki, Hisashi; Nakanishi, Tomoko M; Tanoi, Keitaro.
Affiliation
  • Yang X; Laboratory of Radio Plant Physiology, Department of Applied Biological Chemistry, Graduate School of Agricultural and Life Sciences, The University of Tokyo, Tokyo, Japan.
  • Kobayashi NI; Laboratory of Radio Plant Physiology, Department of Applied Biological Chemistry, Graduate School of Agricultural and Life Sciences, The University of Tokyo, Tokyo, Japan.
  • Hayashi Y; Laboratory of Molecular Genetics, Department of Computational Biology and Medical Sciences, Graduate School of Frontier Sciences, The University of Tokyo, Tokyo, Japan.
  • Ito K; Laboratory of Molecular Genetics, Department of Computational Biology and Medical Sciences, Graduate School of Frontier Sciences, The University of Tokyo, Tokyo, Japan.
  • Moriwaki Y; Bioinformation Engineering Laboratory, Department of Biotechnology, Graduate School of Agricultural and Life Sciences, The University of Tokyo, Tokyo, Japan.
  • Terada T; Collaborative Research Institute for Innovative Microbiology, The University of Tokyo, Tokyo, Japan.
  • Shimizu K; Bioinformation Engineering Laboratory, Department of Biotechnology, Graduate School of Agricultural and Life Sciences, The University of Tokyo, Tokyo, Japan.
  • Hattori M; Collaborative Research Institute for Innovative Microbiology, The University of Tokyo, Tokyo, Japan.
  • Iwata R; Bioinformation Engineering Laboratory, Department of Biotechnology, Graduate School of Agricultural and Life Sciences, The University of Tokyo, Tokyo, Japan.
  • Suzuki H; Collaborative Research Institute for Innovative Microbiology, The University of Tokyo, Tokyo, Japan.
  • Nakanishi TM; State Key Laboratory of Genetic Engineering, Shanghai Key Laboratory of Bioactive Small Molecules, Collaborative Innovation Center of Genetics and Development, Department of Physiology and Neurobiology, School of Life Sciences, Fudan University, Shanghai, China.
  • Tanoi K; Division of Radiopharmaceutical Chemistry, Cyclotron and Radioisotope Center, Tohoku University, Sendai, Japan.
Biosci Biotechnol Biochem ; 86(7): 870-874, 2022 Jun 25.
Article in En | MEDLINE | ID: mdl-35524690
Magnesium is an important nutrient for plants, but much is still unknown about plant Mg2+ transporters. Combining with the structural prediction of AlphaFold2, we used mutagenesis and 28Mg uptake assay to study the highly conserved "GMN" motif of Arabidopsis thaliana MRS2-1 (AtMRS2-1) transporter. We demonstrated that the glycine and methionine in GMN motif are essential for AtMRS2-1 to transport Mg2+.
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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Arabidopsis / Cation Transport Proteins / Arabidopsis Proteins Language: En Journal: Biosci Biotechnol Biochem Journal subject: BIOQUIMICA / BIOTECNOLOGIA Year: 2022 Document type: Article Affiliation country: Japan Country of publication: United kingdom
Fulltext
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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Arabidopsis / Cation Transport Proteins / Arabidopsis Proteins Language: En Journal: Biosci Biotechnol Biochem Journal subject: BIOQUIMICA / BIOTECNOLOGIA Year: 2022 Document type: Article Affiliation country: Japan Country of publication: United kingdom