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Zygote Microinjection for Creating Gene Cassette Knock-in and Flox Alleles in Mice.
Tanimoto, Yoko; Mikami, Natsuki; Ishida, Miyuki; Iki, Natsumi; Kato, Kanako; Sugiyama, Fumihiro; Takahashi, Satoru; Mizuno, Seiya.
Affiliation
  • Tanimoto Y; Laboratory Animal Resource Center and Trans-Border Medical Research Center, University of Tsukuba.
  • Mikami N; Laboratory Animal Resource Center and Trans-Border Medical Research Center, University of Tsukuba; Ph.D. Program in Human Biology, School of Integrative and Global Majors, University of Tsukuba.
  • Ishida M; Laboratory Animal Resource Center and Trans-Border Medical Research Center, University of Tsukuba.
  • Iki N; Laboratory Animal Resource Center and Trans-Border Medical Research Center, University of Tsukuba.
  • Kato K; Laboratory Animal Resource Center and Trans-Border Medical Research Center, University of Tsukuba.
  • Sugiyama F; Laboratory Animal Resource Center and Trans-Border Medical Research Center, University of Tsukuba.
  • Takahashi S; Laboratory Animal Resource Center and Trans-Border Medical Research Center, University of Tsukuba.
  • Mizuno S; Laboratory Animal Resource Center and Trans-Border Medical Research Center, University of Tsukuba; konezumi@md.tsukuba.ac.jp.
J Vis Exp ; (184)2022 06 24.
Article in En | MEDLINE | ID: mdl-35815994
Subject(s)
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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Zygote / CRISPR-Cas Systems Type of study: Prognostic_studies Limits: Animals Language: En Journal: J Vis Exp Year: 2022 Document type: Article
Fulltext
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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Zygote / CRISPR-Cas Systems Type of study: Prognostic_studies Limits: Animals Language: En Journal: J Vis Exp Year: 2022 Document type: Article