Single pot organic solvent-free thermocycling technology for siRNA-ionizable LNPs: a proof-of-concept approach for alternative to microfluidics.

Thermocycling technology is a low-energy, low-temperature, self-assembling cooling­heating process in which lipid droplets spontaneously break apart into much smaller droplets to form siRNA-ionizable LNPs.The new technology is an alternative to multistep, costly, and complex microfluidics technology for the formulation and bulk up of siRNA-ionizable LNPs economically.Thermocycling siRNA-ionizable LNPs formulation focused on optimizing process parameters such as thermal cycle rate, number of thermal cycles, and lipid:aqueous phase ratio.The thermocycling technology is able to overcome the limitations of the storage stability limitations of commercialized ionizable LNPs.