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Immunohistochemical characterization of stem cell, vascular, neural, and differentiation markers in the apical papilla and dental pulp of human teeth at various stages of root development.
Retana-Lobo, Cristina; Reyes-Carmona, Jessie.
Affiliation
  • Retana-Lobo C; Endodontics Section, Restorative Science Department, Faculty of Dentistry, University of Costa Rica, San José, Costa Rica.
  • Reyes-Carmona J; Endodontics Section, Restorative Science Department, Faculty of Dentistry, University of Costa Rica, San José, Costa Rica.
J Histotechnol ; 46(1): 17-27, 2023 03.
Article in En | MEDLINE | ID: mdl-36111541
This study aimed to evaluate the expression of several differentiation markers in the apical papilla (AP) and dental pulp (DP) of human permanent teeth. Twenty young human teeth were extracted and classified according to three Moorrees tooth development stages: initial root formation (Ri), root length ½ (R1/2), and root length complete (Rc). Immunohistochemical assays were performed using STRO-1, VEGF Receptor-2, Neurofilament heavy (NFH), and Nestin antibodies and analyzed under light microscopy. Decalcified, formalin fixed paraffin embedded tooth sections stained with hematoxylin and eosin showed an apical cell rich zone between the DP and AP. The AP revealed fewer vascular and cellular components than the DP. STRO-1 was expressed on vascular and neuronal elements beneath the odontoblast (OB) and in the sub-odontoblastic (SOB) zone, and VEGFR-2 positive cells were observed in the endothelium, arterioles, and blood vessels. Neuroepithelial stem cell protein (Nestin) was highly expressed in differentiated odontoblasts in the predentin odontotoblast and odontoblast cell processes. Neurofilament heavy (NFH) was expressed in mature axons throughout the DP. STRO-1 and VEGFR-2 microvascular expression was higher at the stages Ri and R1/2 while STRO-1 and NFH expression showed strong spatial distribution of Rc neuronal elements as compared to Ri and R1/2. Differentiated OB and SOB cells showed Nestin expression, indicating a reservoir of newly differentiated odontoblast-like cells.
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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Vascular Endothelial Growth Factor Receptor-2 / Dental Pulp Limits: Humans Language: En Journal: J Histotechnol Year: 2023 Document type: Article Affiliation country: Costa Rica Country of publication: United kingdom

Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Vascular Endothelial Growth Factor Receptor-2 / Dental Pulp Limits: Humans Language: En Journal: J Histotechnol Year: 2023 Document type: Article Affiliation country: Costa Rica Country of publication: United kingdom