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Cell wall polysaccharides of endophytic Pseudomonas putida elicit defense against rice blast disease.
Ashajyothi, Mushineni; Balamurugan, Alexander; Patel, Asharani; Krishnappa, Charishma; Kumar, Rajesh; Kumar, Aundy.
Affiliation
  • Ashajyothi M; Division of Plant Pathology, ICAR - Indian Agricultural Research Institute, New Delhi-110012, India.
  • Balamurugan A; Division of Plant Pathology, ICAR - Indian Agricultural Research Institute, New Delhi-110012, India.
  • Patel A; Division of Plant Pathology, ICAR - Indian Agricultural Research Institute, New Delhi-110012, India.
  • Krishnappa C; Division of Plant Pathology, ICAR - Indian Agricultural Research Institute, New Delhi-110012, India.
  • Kumar R; Division of Agricultural Chemicals, ICAR-Indian Agricultural Research Institute, New Delhi-110012, India.
  • Kumar A; Division of Plant Pathology, ICAR - Indian Agricultural Research Institute, New Delhi-110012, India.
J Appl Microbiol ; 134(2)2023 Feb 16.
Article in En | MEDLINE | ID: mdl-36626745
AIMS: To characterize the functional role of extracellular polysaccharides and lipopolysaccharide (LPS) extracted from endophytic Pseudomonas putida BP25 (PpBP25) against rice blast. METHODS AND RESULTS: We profiled the transcriptome of endobacterized rice seedlings using RNA-seq. Fluorescence imaging of interaction between Magnaporthe:: gfp and P. putida:: mCherry was performed on rice phylloplane using confocal laser scanning microscopy (CLSM). Microbial polysaccharides, exopolysaccharide (EPS), and LPS extracted from PpBP25 were characterized using Fourier-transform infrared-spectroscopic analysis (FTIR). Biochemical assays and gene expression analysis were conducted on EPS- and LPS-treated rice seedlings. A detached-leaf assay was designed to test the blasticidal-effect of bacterial-endophyte, EPS, and LPS on rice phylloplane. PpBP25 elicited defense in rice with a consequently altered seedling phenotype. Rice cultivar, Pusa Basmati-1, colonized by PpBP25 showed an altered transcriptome profile displaying a total of 110-downregulated and 68-upregulated genes (P < 0.005) representing growth/development and defense pathways, respectively. CLSM of PpBP25 bacterized phylloplane showed reduced conidial-germination and mycelial-biomass of Magnaporthe oryzae. To decipher the elicitor role of polysaccharides, we purified and characterized EPS and LPS using FTIR. Rice treated with the EPS and LPS showed root-growth inhibition the phenotype of MAMP-triggered immunity. While the EPS showed blast suppressive activity at 1-20 mg mL-1 (79.80%-86.87% reduction over control), the LPS exhibited 78.0%-79.8% reduction at 20-200 µg mL-1on rice. Polysaccharides treated seedling showed elevated activities of peroxidase and polyphenol-oxidase activities, and total-phenols content. Treated plantlets showed up regulation of OsPR1.1,OsPR3, OsGLP3-3,OsZFP179, and Oshox24 as well as downregulation of OsACS6. CONCLUSIONS: We showed that P. putida Bp25 and its cell wall-associated polysaccharides could elicit defense against rice blast.
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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Oryza / Pseudomonas putida / Magnaporthe Aspects: Patient_preference Language: En Journal: J Appl Microbiol Journal subject: MICROBIOLOGIA Year: 2023 Document type: Article Affiliation country: India Country of publication: United kingdom

Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Oryza / Pseudomonas putida / Magnaporthe Aspects: Patient_preference Language: En Journal: J Appl Microbiol Journal subject: MICROBIOLOGIA Year: 2023 Document type: Article Affiliation country: India Country of publication: United kingdom