An antioxidative, green and safe nanofibers-based film containing pullulan, sodium hyaluronate and Ganoderma lucidum fermentation for enhanced skincare.

Dry masks made of natural active ingredients that are packaged in sustainable paper and free of irritating additives (e.g. preservatives, stabilizers) are a trend in the concept of healthy skincare, which possess the advantages of portability, safety and environmental friendliness. The bioactive ingredients obtained from natural plant fermentation are gradually becoming an important alternative additive for facial skincare. Herein, a novel dry facial healthcare mask was fabricated by electrospinning incorporating natural ingredients including pullulan (Pu), sodium hyaluronate (SH), and Ganoderma lucidum fermentation (GLF). The morphology, dissolving capacity, bioactivity, and safety of the obtained masks were investigated in vitro, and their antioxidation and moisturizing activities were verified at the cellular level. The results indicated that the fibrillary films based on pullulan could be dissolved in water within 20 s with good water retention capacity and film with high concentration of GLF (Pu/SH/GLF-3) could scavenge 79 % of DPPH. The films had good ability to resist microbial contamination and non-eye irritation via observing colony growth for 12 months after ultraviolet sterilization and the ocular irritation test of chicken chorioallantoic membrane. Meanwhile, cell experiments further confirmed that they did not exhibit cytotoxicity and could increase the expression of proteins related to moisturizing and antioxidation. The fascinating films have promising application prospects in cosmetic masks. This work may enrich the use of natural materials in skincare products and provide a green development direction for the light chemical industry.