[Effect of high selenium on insulin signaling pathway PI3K-AKT-mTOR in L02 cells].
Wei Sheng Yan Jiu
; 53(1): 77-87, 2024 Jan.
Article
in Zh
| MEDLINE
| ID: mdl-38443176
ABSTRACT
OBJECTIVE:
To observe the effect of high selenium on insulin signaling pathway PI3K-AKT-mTOR in L02 cells.METHODS:
One group of L02 cell was treated with different concentrations of selenomethionine(SeMet, 0.001, 0.0025, 0.005, 0.0075, 0.01, 0.025, 0.05, 0.075 and 0.1µmol/L) for 48 h, then cultured with serum-free medium for 4 h and stimulated with 1 µmol/L insulin for 15 min. The insulin signaling pathway(PI3K-AKT-mTOR) was detected by WB. Another group of L02 cell was treated with the same concentrations of SeMet as above for 48 h. The cell supernatant and lysates were collected for the analysis of SELENOP and GPX1, respectively by WB.RESULTS:
The expressions of P-AKT-(Ser-473), P-AKT-(Thr-308), PI3K and mTOR in L02 cells under high-Se were decreased with the increase of SeMet concentration. The expressions of GPX1 and SELENOP were enhanced with the increase of SeMet.CONCLUSION:
The insulin signaling pathway, PI3K-AKT-mTOR, was damaged in L02 cell under high-Se stress.Key words
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Selenium
Language:
Zh
Journal:
Wei Sheng Yan Jiu
Journal subject:
SAUDE PUBLICA
Year:
2024
Document type:
Article
Affiliation country:
China
Country of publication:
China