Magnetic Polystyrene Nanoparticles Prepared by Emulsion Solvent-Evaporation for the Chemiluminescent Immunoassay.
Chem Pharm Bull (Tokyo)
; 72(3): 340-344, 2024.
Article
in En
| MEDLINE
| ID: mdl-38538315
ABSTRACT
In clinical diagnosis, magnetic polystyrene nanoparticles (MPS NPs) are commonly applied to, e.g., the chemiluminescent immunoassay (CLEIA). However, the conventional preparation method of MPS NPs requires a long duration of heating to form polymer particles, which is inefficient. In this study, we prepared MPS NPs by emulsion solvent-evaporation without heating. We evaluated the effect of the solvent in the water and organic phases on the magnetic particle content. MPS NPs prepared by 4% (v/v) MeOH aqueous solution and adding stearic acid (SA) (4MeSA-MPS NPs) exhibited the highest magnetic particle content. Furthermore, CLEIA analysis indicates that the C-reactive protein detection limit is 80 pg/mL. Thus, 4MeSA-MPS NPs are promising for clinical diagnoses.
Key words
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Polystyrenes
/
Immunoassay
/
Nanoparticles
Language:
En
Journal:
Chem Pharm Bull (Tokyo)
Year:
2024
Document type:
Article
Country of publication:
Japan