Protocol for affinity purification-mass spectrometry interactome profiling in larvae of Drosophila melanogaster.
STAR Protoc
; 5(2): 103064, 2024 Jun 21.
Article
in En
| MEDLINE
| ID: mdl-38743568
ABSTRACT
Many techniques exist for the identification of protein interaction networks. We present a protocol that relies on an affinity purification-mass spectrometry (AP-MS) approach to detect proteins that co-purify with a tagged bait of interest from Drosophila melanogaster larval muscles using the GAL4/upstream activating sequence (UAS) expression system. We also describe steps for the isolation and identification of protein complexes, followed by streamlined bioinformatics analysis for rapid and reproducible results. This protocol can be extended to investigate protein interactions in other tissues. For complete details on the use and execution of this protocol, please refer to Guo et al.1.
Key words
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Mass Spectrometry
/
Drosophila Proteins
/
Drosophila melanogaster
/
Larva
Limits:
Animals
Language:
En
Journal:
STAR Protoc
Year:
2024
Document type:
Article
Country of publication:
United States