Microscopy-based protocol for the quantification of cells viability for temperature-sensitive S. pombe.
STAR Protoc
; 5(2): 103076, 2024 Jun 21.
Article
in En
| MEDLINE
| ID: mdl-38768030
ABSTRACT
Conventional colony-forming unit assay to measure cell viability is laborious and results in large experimental variability, which prohibits accurate quantification of microbial viability. Here, we present a microscopy-based protocol for the quantification of cells viability for temperature-sensitive S. pombe. We describe steps for growing and treating yeast cells and visualization of individual cells viability based on Phloxine B staining. We then detail procedures for data processing using Nikon NIS Elements Advanced Research (AR) software. For complete details on the use and execution of this protocol, please refer to Lim et al.1.
Key words
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Schizosaccharomyces
/
Temperature
Language:
En
Journal:
STAR Protoc
Year:
2024
Document type:
Article