Isolation and target identification of antihepatoma polyprenylphenols from the edible mushroom Suillus granulatus.
Food Funct
; 15(14): 7430-7440, 2024 Jul 15.
Article
in En
| MEDLINE
| ID: mdl-38904163
ABSTRACT
Eight polyprenylphenol derivatives were isolated from the wild edible mushroom Suillus granulatus, including seven novel compounds, named suillin F-L (2-8), and one previously identified compound (1). The structures of the new compounds were elucidated using HR-ESI-MS and 1D and 2D NMR data. The absolute configuration of compound 8 was assigned based on the comparison of the experimental and calculated ECD data. All isolated compounds were evaluated for their cytotoxicity against HepG2 cancer cell lines. Compounds 1 and 3-6 demonstrated significant antitumor activity compared to the positive control (cisplatin), with IC50 values ranging from 8.19 to 13.97 µM. Furthermore, DARTS assay and LC-MS/MS analysis were used to identify HSP90AA1 as the direct target of compound 5, and the interaction between compound 5 and HSP90AA1 was verified by molecular docking.
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Phenols
/
Molecular Docking Simulation
/
Antineoplastic Agents
Limits:
Humans
Language:
En
Journal:
Food Funct
Year:
2024
Document type:
Article
Affiliation country:
China
Country of publication:
United kingdom