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Chemical characterization, in vivo safety profile and effects of the extract from unconventional food plant Acca sellowiana (O. BERG) Burret on endothelial cells under glucose overload.
Minello, Luana; Mattiello, Gabrielle Slomp; Cassini, Carina; Touguinha, Luciana Andrade; Paim, Luís Fernando; Moura, Sidnei; Salvador, Mirian; Branco, Catia Santos.
Affiliation
  • Minello L; Laboratory of Oxidative Stress and Antioxidants, Institute of Biotechnology, University of Caxias do Sul, Caxias do Sul, RS, Brazil.
  • Mattiello GS; Laboratory of Oxidative Stress and Antioxidants, Institute of Biotechnology, University of Caxias do Sul, Caxias do Sul, RS, Brazil.
  • Cassini C; Laboratory of Oxidative Stress and Antioxidants, Institute of Biotechnology, University of Caxias do Sul, Caxias do Sul, RS, Brazil.
  • Touguinha LA; Laboratory of Oxidative Stress and Antioxidants, Institute of Biotechnology, University of Caxias do Sul, Caxias do Sul, RS, Brazil.
  • Paim LF; Laboratory of Biotechnology, Natural and Synthetic Products, Institute of Biotechnology, University of Caxias do Sul, Caxias do Sul, RS, Brazil.
  • Moura S; Laboratory of Biotechnology, Natural and Synthetic Products, Institute of Biotechnology, University of Caxias do Sul, Caxias do Sul, RS, Brazil.
  • Salvador M; Laboratory of Oxidative Stress and Antioxidants, Institute of Biotechnology, University of Caxias do Sul, Caxias do Sul, RS, Brazil.
  • Branco CS; Laboratory of Oxidative Stress and Antioxidants, Institute of Biotechnology, University of Caxias do Sul, Caxias do Sul, RS, Brazil. Electronic address: csbranc1@ucs.br.
J Pharm Biomed Anal ; 248: 116304, 2024 Sep 15.
Article in En | MEDLINE | ID: mdl-38906070
ABSTRACT
"Guava" (Acca sellowiana) is an unconventional edible plant from Brazil. It is used in traditional medicine as an anti-diabetic; however, pharmacological studies on this plant are scarce. This study aimed to evaluate the chemical and safety profile of an aqueous A. sellowiana peel extract (ASPE) and its effects on endothelial EA.hy926 cells under glucose overload and in vivo (Artemia salina). An ethanolic extract from A. sellowiana peels (ASPEetOH) was also produced and characterized. Results showed that ASPE did not present in vivo toxicity, and it was found to contain high phenolic content and redox capacity. ASPE (50 µg/mL; 24 h) prevented oxidative stress and mitochondrial dysfunction, besides positively modulating Sirtuins 1 and 3, and prevented the increase of COX-2 and NF-kß expression levels in EA.hy926 cells under glucose overload. Chromatographic fractionation, metabolite profiling, spectroscopic and bioinformatics analyses revealed the presence of phenolic acids, flavan-3-ols, flavonols, flavones, flavanones, and anthocyanidins, displaying a diversity of compounds in the crude and fractionated ASPEetOH. This study provided evidence on the safety profile, chemical composition, and pharmacological activities of A. sellowiana.
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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Plant Extracts / Endothelial Cells / Glucose Limits: Animals / Humans Country/Region as subject: America do sul / Brasil Language: En Journal: J Pharm Biomed Anal Year: 2024 Document type: Article Country of publication: United kingdom
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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Plant Extracts / Endothelial Cells / Glucose Limits: Animals / Humans Country/Region as subject: America do sul / Brasil Language: En Journal: J Pharm Biomed Anal Year: 2024 Document type: Article Country of publication: United kingdom