Defining context-dependent m6A RNA methylomes in Arabidopsis.
Dev Cell
; 2024 Jul 10.
Article
in En
| MEDLINE
| ID: mdl-39025060
ABSTRACT
N6-Methyladenosine (m6A) prevalently occurs on cellular RNA across almost all kingdoms of life. It governs RNA fate and is essential for development and stress responses. However, the dynamic, context-dependent m6A methylomes across tissues and in response to various stimuli remain largely unknown in multicellular organisms. Here, we generate a comprehensive census that identifies m6A methylomes in 100 samples during development or following exposure to various external conditions in Arabidopsis thaliana. We demonstrate that m6A is a suitable biomarker to reflect the developmental lineage, and that various stimuli rapidly affect m6A methylomes that constitute the regulatory network required for an effective response to the stimuli. Integrative analyses of the census and its correlation with m6A regulators identify multiple layers of regulation on highly context-dependent m6A modification in response to diverse developmental and environmental stimuli, providing insights into m6A modification dynamics in the myriad contexts of multicellular organisms.
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Language:
En
Journal:
Dev Cell
Journal subject:
EMBRIOLOGIA
Year:
2024
Document type:
Article
Affiliation country:
Singapore
Country of publication:
United States