Identification of attractants for adult Spodoptera litura based on the interaction between odorant-binding protein 34 and host volatiles.
Pestic Biochem Physiol
; 203: 106005, 2024 Aug.
Article
in En
| MEDLINE
| ID: mdl-39084800
ABSTRACT
Odorant-binding proteins (OBPs) play key roles in host plant location by insects, and can accordingly serve as important targets for the development of attractants. In this study, we detected the high expression of SlitOBP34 in male antennae of Spodoptera litura. Subsequently, the fluorescence competitive binding experiments displayed that the SlitOBP34 protein has binding affinity for different ligands. Then, protein-ligand interaction analyses found the presence of six amino acid residues may serve as key recognition sites. Further electroantennographic and biobehavioral assessments revealed that the electrophysiological responses of male antennae were evoked in response to stimulation with the six identified host volatiles, and that these volatiles attracted male moths to varying extents. Notably, low concentrations of benzaldehyde, 1-hexanol, and cis-3-hexenyl acetate were found to have significant attractant effects on male moths, thereby identifying these three host volatiles as potential candidates for the development of male attractants. These findings advance our current understanding of the olfactory-encoded mechanisms of host plants selection in S. litura and have enabled us to develop novel adult attractants for controlling the pest in the future.
Key words
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Receptors, Odorant
/
Spodoptera
/
Insect Proteins
/
Volatile Organic Compounds
/
Arthropod Antennae
Limits:
Animals
Language:
En
Journal:
Pestic Biochem Physiol
Year:
2024
Document type:
Article
Affiliation country:
China
Country of publication:
United States