Your browser doesn't support javascript.
loading
Generation of green fluorescent protein reporter knock-in iPSC line at the 3'UTR region of the KLOTHO locus.
Lim, Sun Woo; Lee, Kang In; Cui, Sheng; Fang, Xianying; Shin, Yoo Jin; Lee, Hanbi; Lee, Jae Young; Chung, Byung Ha; Yang, Chul Woo.
Affiliation
  • Lim SW; Transplantation Research Center, College of Medcine, The Catholic University of Korea, Seoul, Republic of Korea.
  • Lee KI; R&D Center, ToolGen, Inc., Seoul, Republic of Korea.
  • Cui S; Transplantation Research Center, College of Medcine, The Catholic University of Korea, Seoul, Republic of Korea.
  • Fang X; Transplantation Research Center, College of Medcine, The Catholic University of Korea, Seoul, Republic of Korea.
  • Shin YJ; Transplantation Research Center, College of Medcine, The Catholic University of Korea, Seoul, Republic of Korea.
  • Lee H; Transplantation Research Center, College of Medcine, The Catholic University of Korea, Seoul, Republic of Korea; Department of Internal Medicine, Seoul St. Mary's Hospital, The Catholic University of Korea, Seoul, Republic of Korea.
  • Lee JY; R&D Center, ToolGen, Inc., Seoul, Republic of Korea.
  • Chung BH; Transplantation Research Center, College of Medcine, The Catholic University of Korea, Seoul, Republic of Korea; Department of Internal Medicine, Seoul St. Mary's Hospital, The Catholic University of Korea, Seoul, Republic of Korea. Electronic address: chungbh@catholic.ac.kr.
  • Yang CW; Transplantation Research Center, College of Medcine, The Catholic University of Korea, Seoul, Republic of Korea; Department of Internal Medicine, Seoul St. Mary's Hospital, The Catholic University of Korea, Seoul, Republic of Korea. Electronic address: yangch@catholic.ac.kr.
Stem Cell Res ; 80: 103499, 2024 Oct.
Article in En | MEDLINE | ID: mdl-39111000
ABSTRACT
We generated a human induced pluripotent stem cell (hiPSC) line (CMCi014-A-78) expressing a GFP reporter in the 3'-UTR region of the KLOTHO locus using CRISPR/Cas9-mediated homologous recombination to screen for candidates regulating KLOTHO. The established cell line exhibits a normal karyotype, typical stem cell morphology, expression of pluripotency markers, and the ability to differentiate into the three germ layers. Consequently, this hiPSC line could serve as a valuable resource for screening KLOTHO regulators in hiPSC-derived target cells or organoids.
Subject(s)
Fulltext
Add to My VHL
Print
XML
PubMed Links
Search on Google

Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: 3' Untranslated Regions / Green Fluorescent Proteins / Induced Pluripotent Stem Cells / Klotho Proteins / Glucuronidase Limits: Humans Language: En Journal: Stem Cell Res Year: 2024 Document type: Article Country of publication: United kingdom
Fulltext
Add to My VHL
Print
XML
PubMed Links
Search on Google

Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: 3' Untranslated Regions / Green Fluorescent Proteins / Induced Pluripotent Stem Cells / Klotho Proteins / Glucuronidase Limits: Humans Language: En Journal: Stem Cell Res Year: 2024 Document type: Article Country of publication: United kingdom