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Proteomic Analysis of Secreted Vesicle Proteins from the Plant Cells.
Yin, Haidi; Wang, Jianying; Yao, Zhong-Ping.
Affiliation
  • Yin H; Multi-omics Mass Spectrometry Facility, Shenzhen Medical Academy of Research and Translation, Shenzhen, Guangdong, China.
  • Wang J; Department of Applied Biology and Chemical Technology, The Hong Kong Polytechnic University, Hung Hom, Kowloon, Hong Kong, China.
  • Yao ZP; Department of Applied Biology and Chemical Technology, The Hong Kong Polytechnic University, Hung Hom, Kowloon, Hong Kong, China. zhongping.yao@polyu.edu.hk.
Methods Mol Biol ; 2841: 67-73, 2024.
Article in En | MEDLINE | ID: mdl-39115765
ABSTRACT
A working pipeline for proteomic analysis of secreted vesicle proteins from the plant cells has been developed using urea and mass spectrometry-compatible detergent RapiGest SF, where vesicles could be efficiently lysed and membrane-bound proteins could be efficiently dissolved and digested. The vesicle lysis and the protein digestion procedures are performed within one tube to minimize the protein loss. The protein digest is analyzed using LC-MS/MS after desalting with an SPE spin column.
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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Plant Proteins / Proteomics / Tandem Mass Spectrometry / Plant Cells Language: En Journal: Methods Mol Biol Journal subject: BIOLOGIA MOLECULAR Year: 2024 Document type: Article Affiliation country: China Country of publication: United States
Fulltext
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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Plant Proteins / Proteomics / Tandem Mass Spectrometry / Plant Cells Language: En Journal: Methods Mol Biol Journal subject: BIOLOGIA MOLECULAR Year: 2024 Document type: Article Affiliation country: China Country of publication: United States