Development of a 3-dimensional organotypic model with characteristics of peripheral sensory nerves.
Cell Rep Methods
; 4(8): 100835, 2024 Aug 19.
Article
in En
| MEDLINE
| ID: mdl-39116883
ABSTRACT
We developed a rat dorsal root ganglion (DRG)-derived sensory nerve organotypic model by culturing DRG explants on an organoid culture device. With this method, a large number of organotypic cultures can be produced simultaneously with high reproducibility simply by seeding DRG explants derived from rat embryos. Unlike previous DRG explant models, this organotypic model consists of a ganglion and an axon bundle with myelinated A fibers, unmyelinated C fibers, and stereo-myelin-forming nodes of Ranvier. The model also exhibits Ca2+ signaling in cell bodies in response to application of chemical stimuli to nerve terminals. Further, axonal transection increases the activating transcription factor 3 mRNA level in ganglia. Axons and myelin are shown to regenerate 14 days following transection. Our sensory organotypic model enables analysis of neuronal excitability in response to pain stimuli and tracking of morphological changes in the axon bundle over weeks.
Key words
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Axons
/
Ganglia, Spinal
/
Microphysiological Systems
Limits:
Animals
Language:
En
Journal:
Cell Rep Methods
/
Cell reports. Methods
Year:
2024
Document type:
Article
Affiliation country:
Japan
Country of publication:
United States