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Risk assessment of 2ß,3ß-19α-trihydroxyursolic acid from Rubus imperialis (Rosaceae) in HepG2/C3A cells via genotoxicity, metabolism, and cell growth.
Oshiiwa, Bruna; da Silva, Aline Pereira; Alves, Greice Rafaele; Filho, Valdir Cechinel; Niero, Rivaldo; O'Neill de Mascarenhas Gaivão, Isabel; de Oliveira, Liana Martins; de Lima, Luan Vitor Alves; Mantovani, Mário Sérgio; Maistro, Edson Luis.
Affiliation
  • Oshiiwa B; Faculty of Philosophy and Sciences, Speech and Hearing Therapy Department, São Paulo State University (UNESP), Marília, São Paulo State, Brazil.
  • da Silva AP; Postgraduate Program in Health and Aging, Marilia Medical School (FAMEMA), Marilia, São Paulo State, Brazil.
  • Alves GR; Programa de Pós-Graduação em Ciências Farmacêuticas e Núcleo de Investigações Químico-Farmacêuticas (NIQFAR), Vale do Itajaí University (UNIVALI), Itajaí, Santa Catarina State, Brazil.
  • Filho VC; Programa de Pós-Graduação em Ciências Farmacêuticas e Núcleo de Investigações Químico-Farmacêuticas (NIQFAR), Vale do Itajaí University (UNIVALI), Itajaí, Santa Catarina State, Brazil.
  • Niero R; Programa de Pós-Graduação em Ciências Farmacêuticas e Núcleo de Investigações Químico-Farmacêuticas (NIQFAR), Vale do Itajaí University (UNIVALI), Itajaí, Santa Catarina State, Brazil.
  • O'Neill de Mascarenhas Gaivão I; CECAV and Department of Genetics and Biotechnology, University of Trás-os-Montes and Alto Douro, Vila Real, Portugal.
  • de Oliveira LM; Department of General Biology, Londrina State University (UEL), Londrina, Paraná State, Brazil.
  • de Lima LVA; Department of General Biology, Londrina State University (UEL), Londrina, Paraná State, Brazil.
  • Mantovani MS; Department of General Biology, Londrina State University (UEL), Londrina, Paraná State, Brazil.
  • Maistro EL; Faculty of Philosophy and Sciences, Speech and Hearing Therapy Department, São Paulo State University (UNESP), Marília, São Paulo State, Brazil.
J Appl Toxicol ; 2024 Aug 11.
Article in En | MEDLINE | ID: mdl-39128859
ABSTRACT
Rubus imperialis (Rosaceae) is a Brazilian medicinal plant that already exhibited therapeutical perspectives. However, previous studies revealed cellular and/or genetic toxicity of extracts from aerial parts of this plant, as well as other species of the Rubus genus. Being 2ß,3ß-19α-trihydroxyursolic acid (2B) one of the major compounds of this plant, with proven pharmacological effect, it is important to investigate the biosafety of this isolated compound. Therefore, in the present study, (2B) was tested by several cytogenotoxic endpoints up to 20 µg/ml in human hepatoma HepG2/C3A cells. The test compound did not produce any decreased cell viability, DNA damage, chromosomal mutations, cell cycle changes, or apoptotic effects in the tested cells. Additionally, RT-qPCR analysis revealed the downregulation of CYP3A4 (metabolism), M-TOR (cell death), and CDKN1A (cell cycle) genes. Under the experimental conditions used, the 2B compound did not show cytogenotoxic activity after a single exposure to HepG2/C3A human cells.
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Full text: 1 Collection: 01-internacional Database: MEDLINE Language: En Journal: J Appl Toxicol / J. appl. toxicol / Journal of applied toxicology Year: 2024 Document type: Article Affiliation country: Brazil Country of publication: United kingdom
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Full text: 1 Collection: 01-internacional Database: MEDLINE Language: En Journal: J Appl Toxicol / J. appl. toxicol / Journal of applied toxicology Year: 2024 Document type: Article Affiliation country: Brazil Country of publication: United kingdom