Translation in Bacillus subtilis is spatially and temporally coordinated during sporulation.
Nat Commun
; 15(1): 7188, 2024 Aug 21.
Article
in En
| MEDLINE
| ID: mdl-39169056
ABSTRACT
The transcriptional control of sporulation in Bacillus subtilis is reasonably well understood, but its translational control is underexplored. Here, we use RNA-seq, ribosome profiling and fluorescence microscopy to study the translational dynamics of B. subtilis sporulation. We identify two events of translation silencing and describe spatiotemporal changes in subcellular localization of ribosomes during sporulation. We investigate the potential regulatory role of ribosomes during sporulation using a strain lacking zinc-independent paralogs of three zinc-dependent ribosomal proteins (L31, L33 and S14). The mutant strain exhibits delayed sporulation, reduced germination efficiency, dysregulated translation of metabolic and sporulation-related genes, and disruptions in translation silencing, particularly in late sporulation.
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Ribosomal Proteins
/
Ribosomes
/
Spores, Bacterial
/
Bacillus subtilis
/
Bacterial Proteins
/
Protein Biosynthesis
/
Gene Expression Regulation, Bacterial
Language:
En
Journal:
Nat Commun
Journal subject:
BIOLOGIA
/
CIENCIA
Year:
2024
Document type:
Article
Affiliation country:
Poland
Country of publication:
United kingdom