Oxidation of Cyclohexane to Cyclohexanol/Cyclohexanone Using Sol-Gel-Encapsulated Unspecific Peroxygenase from Agrocybe aegerita.
ChemistryOpen
; 13(10): e202400152, 2024 Oct.
Article
in En
| MEDLINE
| ID: mdl-39212291
ABSTRACT
Unspecific peroxygenase from Agrocybe aegerite (AaeUPO) is a remarkable catalyst for the oxyfunctionalization of non-activated C-H bonds under mild conditions. It exhibits comparable activity to P450 monooxygenase but offers the advantage of using H2O2 instead of a complex electron transport chain to reductively activate O2. Here, we demonstrate the successful oxidation of cyclohexane to cyclohexanol/cyclohexanone (KA-oil) using sol-gel encapsulated AaeUPO. Remarkably, cyclohexane serves both as a solvent and a substrate in this system, which simplifies product isolation. The ratio of cyclohexanone to cyclohexanol using this approach is remarkably higher compared to the oxidation using free AaeUPO in aqueous media using acetonitrile as a cosolvent. The utilization of sol-gel encapsulated AaeUPO offers a promising approach for oxyfunctionalization reactions and improves the chances for this enzyme to be incorporated in the same pot with other chemical transformations.
Key words
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Oxidation-Reduction
/
Cyclohexanes
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Cyclohexanols
/
Cyclohexanones
/
Agrocybe
/
Mixed Function Oxygenases
Language:
En
Journal:
ChemistryOpen
Year:
2024
Document type:
Article
Affiliation country:
Netherlands
Country of publication:
Germany