Comprehensive transcriptome analysis of Asparagus officinalis in response to varying levels of salt stress.

ABSTRACT

BACKGROUND: Salt stress is a major abiotic factor that affects the distribution and growth of plants. Asparagus officinalis is primarily resistant to salt stress and is suitable for cultivation in saline-alkali soil. RESULTS: The study integrated the morphology, physiological indexes, and transcriptome of A. officinalis exposed to different levels of NaCl, with the aim of understanding its biological processes under salt stress. The findings indicated that exposure to salt stress led to decreases in the height and weight of A. officinalis plants. Additionally, the levels of POD and SOD, as well as the amounts of MDA, proline, and soluble sugars, showed an increase, whereas the chlorophyll content decreased. Analysis of the transcriptome revealed that 6,203 genes that showed differential expression at different salt-stress levels. Various TFs, including FAR1, MYB, NAC, and bHLH, exhibited differential expression under salt stress. KEGG analysis showed that the DEGs were primarily associated with the plant hormone signal transduction and lignin biosynthesis pathways. CONCLUSION: These discoveries provide a solid foundation for an in-depth exploration of the pivotal genes, including Aux/IAA, TCH4, COMT, and POD, among others, as well as the pathways involved in asparagus's salt stress responses. Consequently, they have significant implications for the future analysis of the molecular mechanisms underlying asparagus's response to salt stress.