Assessment of transthyretin instability in patients with wild-type transthyretin amyloid cardiomyopathy.
Sci Rep
; 14(1): 20508, 2024 09 03.
Article
in En
| MEDLINE
| ID: mdl-39227655
ABSTRACT
The pathophysiology of variant transthyretin (TTR) amyloidosis (ATTRv) is associated with destabilizing mutations in the TTR tetramer. However, why TTR with a wild-type genetic sequence misfolds and aggregates in wild-type transthyretin amyloidosis (ATTRwt) is unknown. Here, we evaluate kinetic TTR stability with a newly developed ELISA system in combination with urea-induced protein denaturation. Compared with that in control patients, endogenous TTR in patients with wild-type transthyretin amyloid cardiomyopathy (ATTRwt-CM) exhibited thermodynamic instability, indicating that circulating TTR instability may be associated with the pathogenesis of ATTRwt as well as ATTRv. Our findings provide new insight into the underlying mechanisms of ATTRwt.
Key words
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Prealbumin
/
Amyloid Neuropathies, Familial
/
Cardiomyopathies
Limits:
Aged
/
Female
/
Humans
/
Male
/
Middle aged
Language:
En
Journal:
Sci Rep
Year:
2024
Document type:
Article
Affiliation country:
Japan
Country of publication:
United kingdom