Direct or DNA Extraction-Free Amplification and Quantification of Foodborne Pathogens.
Methods Mol Biol
; 2852: 3-17, 2025.
Article
in En
| MEDLINE
| ID: mdl-39235733
ABSTRACT
The use of direct nucleic acid amplification of pathogens from food matrices has the potential to reduce time to results over DNA extraction-based approaches as well as traditional culture-based approaches. Here we describe protocols for assay design and experiments for direct amplification of foodborne pathogens in food sample matrices using loop-mediated isothermal amplification (LAMP) and polymerase chain reaction (PCR). The examples provided include the detection of Escherichia coli in milk samples and Salmonella in pork meat samples. This protocol includes relevant reagents and methods including obtaining target sequences, assay design, sample processing, and amplification. These methods, though used for specific example matrices, could be applied to many other foodborne pathogens and sample types.
Key words
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Salmonella
/
DNA, Bacterial
/
Polymerase Chain Reaction
/
Nucleic Acid Amplification Techniques
/
Milk
/
Food Microbiology
Limits:
Animals
Language:
En
Journal:
Methods Mol Biol
Journal subject:
BIOLOGIA MOLECULAR
Year:
2025
Document type:
Article
Affiliation country:
United States
Country of publication:
United States