Salidroside prevents cadmium chloride-induced DNA damage in human fetal lung fibroblasts.
J Trace Elem Med Biol
; 86: 127521, 2024 Sep 05.
Article
in En
| MEDLINE
| ID: mdl-39243731
ABSTRACT
BACKGROUND:
Cadmium (Cd) is an environmental pollutant and a heavy metal known for its genotoxic effects, which can lead to cancer and other related diseases. Preventing Cd-induced genotoxicity is crucial; however, there is limited research on this topic. Salidroside (SAL), a phenylpropanoid glycoside isolated from Rhodiola rosea L., is a popular medicinal compound with several health benefits. Nevertheless, its therapeutic effect on Cd-induced genotoxicity remains unexplored.METHODS:
Human fetal lung fibroblasts were treated with 20⯵M Cd2+ (CdCl2) for 12â¯h and 5-20⯵M SAL was used to test the anti-DNA damage effect. DNA damage was evaluated using γH2AX expression and the alkaline comet assay. Intracellular reactive oxygen species (ROS) levels were measured using flow cytometry.RESULTS:
Exposure to 20⯵M Cd2+ for 12â¯h induced significant DNA damage in human fetal lung fibroblasts, and this effect was notably attenuated by SAL treatment. SAL treatment did not decrease ROS levels in cells treated with Cd2+.CONCLUSION:
SAL effectively prevented Cd2+-induced DNA damage in human fetal lung fibroblasts. However, the underlying mechanism requires further investigation.
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Language:
En
Journal:
J Trace Elem Med Biol
/
J. trace elem. med. biol
/
Journal of trace elements in medicine and biology
Journal subject:
METABOLISMO
/
SAUDE AMBIENTAL
Year:
2024
Document type:
Article
Country of publication:
Germany