A primer on single-cell RNA-seq analysis using dendritic cells as a case study.
FEBS Lett
; 2024 Sep 08.
Article
in En
| MEDLINE
| ID: mdl-39245787
ABSTRACT
Recent advances in single-cell (sc) transcriptomics have revolutionized our understanding of dendritic cells (DCs), pivotal players of the immune system. ScRNA-sequencing (scRNA-seq) has unraveled a previously unrecognized complexity and heterogeneity of DC subsets, shedding light on their ontogeny and specialized roles. However, navigating the rapid technological progress and computational methods can be daunting for researchers unfamiliar with the field. This review aims to provide immunologists with a comprehensive introduction to sc transcriptomic analysis, offering insights into recent developments in DC biology. Addressing common analytical queries, we guide readers through popular tools and methodologies, supplemented with references to benchmarks and tutorials for in-depth understanding. By examining findings from pioneering studies, we illustrate how computational techniques have expanded our knowledge of DC biology. Through this synthesis, we aim to equip researchers with the necessary tools and knowledge to navigate and leverage scRNA-seq for unraveling the intricacies of DC biology and advancing immunological research.
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Language:
En
Journal:
FEBS Lett
Year:
2024
Document type:
Article
Affiliation country:
Italy
Country of publication:
United kingdom